Antimicrobial activity of green tea extract against isolates of methicillin-resistant Staphylococcus aureus and multi-drug resistant Pseudomonas aeruginosa

作     者：Maksum Radji Rafael Adi Agustama Berna Elya Conny Riana Tjampakasari 

作者机构：Laboratory of Microbiology and BiotechnologyFaculty of PharmacyUniversity of IndonesiaDepok16424Indonesia Laboratory of PhytochemistryFaculty of PharmacyUniversity of IndonesiaDepok 16424Indonesia Department of MicrobiologyMedical FacultyUniversity of IndonesiaJakarta 10320Indonesia 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2013年第3卷第8期

页      面：663-667页

学科分类：1007[医学-药学(可授医学、理学学位)] 100705[医学-微生物与生化药学] 1001[医学-基础医学(可授医学、理学学位)] 100103[医学-病原生物学] 10[医学] 

基　　金：Supported by Collaborative Project Research Faculty of Pharmacy and Department of Microbiology Medical Faculty University of Indonesia Grant No.2012/0806327660 

主　　题：Camellia sinensis Green tea Antibacterial activity MIC Multi-drug resistant bacteria 

摘      要：Objective:To evaluate antibacterial activity of the Indonesian water soluble green tea extract,Camellia sinensis,against clinical isolates of methicillin-resistantStaphylococcus aureus (***)(MRSA)and multi-drug resistant Pseudomonas aeruginosa(***).Methods:Antimicrobial activity of green tea extract was determined by the disc diffusion method and the minimum inhibitory concentration(MIC)was determined by the twofold serial broth dilutions *** tested bacteria using in this study were the standard strains and multi-drug resistant clinical isolates of *** and ***,obtained from Laboratory of Clinical Microbiology,Faculty of Medicine,University of ***:The results showed that the inhibition zone diameter of green tea extracts *** ATCC 25923 and MRSA were(18.970依0.287)mm,and(19.130依0.250)mm *** the inhibition zone diameter *** ATCC 27853 and ***(17.550依0.393)mm and(17.670依0.398)mm *** MIC of green tea extracts *** ATCC 25923 and MRSA were 400μg/mL and 400μg/mL,respectively,whereas the MIC for *** ATCC 27853 and *** 800μg/mL,and 800μg/mL,***:Camellia sinensisleaves extract could be useful in combating emerging drug-resistance caused by MRSA ***.