Genome-wide mapping of QTL associated with heterosis in the RIL-based NCIII design

作     者：HE XiaoHong HU ZhongLi ZHANG Yuan-Ming 

作者机构：Section on Statistical GenomicsState Key Laboratory of Crop Genetics and Germplasm Enhancement&Chinese National Center for Soybean ImprovementCollege of AgricultnreNanjing Agricultural UniversityNanjing 210095China State Key Laboratory of Hybrid RiceCollege of Life SciencesWuhan UniversityWuhan 430072China 

出 版 物：《Chinese Science Bulletin》 (中国科学通报)

年 卷 期：2012年第57卷第21期

页      面：2655-2665页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 08[工学] 09[农学] 071007[理学-遗传学] 0901[农学-作物学] 0836[工学-生物工程] 090102[农学-作物遗传育种] 

基　　金：supported by the National Basic Research Program of China(2011CB109306) a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions the National Natural Science Foundation of China(30900842,31000666) the NAU Youth Sci-Tech Innovation Fund(KJ08001) 

主　　题：数量性状位点 全基因组 杂种优势 设计 测绘 上位性效应 统计方法 复合区间作图 

摘      要：Heterosis represents one of the most revolutionary advancements in crop *** the genetic dissection of heterosis,NCIII design is one of the most powerful and widely used mating ***,the methodologies for quantitative trait loci (QTL) detection in the design were mostly based on composite interval ***,in this study,our purpose was to develop a statistical method for mapping epistatic QTL associated with heterosis in the RIL-based NCIII ***,we derivated the expectations of two classical linear transformations,Z 1 and Z 2,while a quantitative trait was controlled by two QTL with digenic epistasis and arbitrary linkage under the F ∞ and F 2 metric ***,we constructed an epistatic genetic model that includes all markers on the whole genome simultaneously,and estimated all the parameters in the model by the empirical Bayes ***,a series of Monte Carlo simulation experiments was carried out to confirm the proposed *** results show that:(1) all the augmented genetic parameters for main-effect QTL could be rightly identified with satisfactory statistical power and precision;(2) the statistical powers in the detection of augmented epistatic effects were substantively affected by the signs of pure epistatic effects;(3) it is more difficult to detect epistatic QTL than to detect main-effect QTL;(4) statistical power is higher in the RIL-based NCIII design than in the F 2-based NCIII design,especially in the detection of the augmented epistatic effect that consists of two pure epistatic effects in opposite directions.