Novel Kdr mutations (K964R and A943V) in pyrethroid-resistant populations of Triatoma mazzottii and Triatoma longipennisUoro Mexico and detoxifying enzymes

作     者：Jesus Davila-Barboza O.Karina Villanueva-Segura Beatriz Lopez-Monroy Gustavo Ponce-Garcia Cristina Bobadilla-Utrera Mayela Montes-Rincon Zinnia J.Molina-Garza Juan I.Arredondo-Jimenez Iram P.Rodriguez-Sanchez Pablo C.Manrique-Saide Adriana E.Flores 

作者机构：Universidad Autonoma de Nuevo Leon (UANL)Facultad de Ciencias BiologicasSan Nicolas de los GarzaNLMexico Laboratorio Estatal de Salud Publica de los Servicios de Salud de VeracruzVeracruzMexico Campus de Ciencias Biologicas y AgropecuariasUnidad Colaborativa para Bioensayos EntomologicosUniversidad Autonoma de YucatanMeridaYucatanMexico 

出 版 物：《Insect Science》 (昆虫科学（英文版）)

年 卷 期：2019年第26卷第5期

页      面：809-820页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

主　　题：detoxifying enzymes kdr pyrethroids Triatoma longipennis Triatoma mazzottii 

摘      要：Although having five different ways of transmission the vector-borne is the principal way of transmission of Chagas disease, which involves insects of the subfamily Triatominae (Hemiptera: Reduviidae). Nineteen of the 31 species that occur in Mexico are associated with humans, and all are capable of transmitting the disease. Pyrethroids are the insecticides recommended for the control of these vectors in Mexico. We determined the susceptibility to the pyrethroids dcltamethrin and permethrin of peridomestic populations of Triatoma mazzottii Usinger and two populations of Triatoma longipetmis Usinger in comparison with a reference strain for each species. Bioassays were performed for the determination of the LD50 for both field populations and reference strains. A maximum of 27 fold resistance to deltamethrin was observed in T. mazzottii, meanwhile, for permethrin, T. longipennis from Jalisco show the highest value of 3.19 fold. There was significantly increased activity of esterases in field populations in comparison with their corresponding reference strain. The results of the search of kdr mutations related to the resistance to deltamethrin and permethrin in the evaluated species show the presence of mutations in the field populations, as is the case with individuals of T. mazzottii, for which the mutation was found A943V, and for the two populations of T. longipennis included in this study, we report the presence of the kdr mutation K964R. Evaluation of the various mechanisms involved in resistance to pyrethroids in triatomines from Mexico could guide us to the real justification for insecticide resistance monitoring.