Bcl-2shRNA转染联合塞莱昔布对胃癌细胞株SGC-7901的影响(英文)

作     者：Weiwei Liu Aiqin Wang Wenjing Xiao Fangzhen Shen Minggang Lin Ruyong Yao 

作者机构：Department of OncologyThe Affiliated Hospital of Medical College Qingdao UniversityQingdao 266003China 

出 版 物：《The Chinese-German Journal of Clinical Oncology》 (中德临床肿瘤学杂志（英文版）)

年 卷 期：2012年第11卷第2期

页      面：104-108页

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 1002[医学-临床医学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

主　　题：shRNA Bcl-2 细胞转染 细胞株 ELISA法 siRNA 生长抑制率 流式细胞仪 

摘      要：Objective:The aim of this study was to study the changes of SGC-7901 cells transfecting small hairpin RNA(shRNA) targeted Bcl-2 and celecoxib in ***:To use the effective siRNA targeted to Bcl-2 by Lipofectamine 2000,the rate of cell growth inhibition of all groups was detected by multiply-table tournament(MTT) method,apoptosis rate was examined by flow cytometry and the expression of Bcl-2 was assayed by ***:After siRNA was transfected to SGC-7901 cells,the rate of cell growth inhibition was increased,the joint group was higher by flow cytometry and the expression of Bcl-2 was lower by ***:The growth of SGC-7901 cells which was transfected siRNA has been inhibited,and the sensitivity to celecoxib has been increased.