MOLECULAR ALTERATION OF α-INDUCED TRANSFORMED SYRIAN HAMSTER EMBRYO CELLS

作     者：寿江 章扬培 吴德昌 

作者机构：Institute of Radiation Medicine Beijing 100850. 

出 版 物：《Chinese Medical Sciences Journal》 (中国医学科学杂志（英文版）)

年 卷 期：1997年第12卷第1期

页      面：6-10页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 071008[理学-发育生物学] 071007[理学-遗传学] 

主　　题：a particles cell transformation oncogenes 

摘      要：Syrian hamster ernbryo(SHE) cells are irradiated by a particles emitted from Plutonium-238 in *** frequencies of donor DNA derived from passaged cells,including monocloned cells from 20th passage cells,after irradiation are parallel with their tumorigenicity of donor cells. After trans formation, expressions of H-ras and c-myc genes are in the state of activation, which might be resulted from occurrence of mutation in H-ras gene and hypomethylation of c-myc gene respectively, even though no alteration was observed on their restriction fragment length polymorphisms(RELP) patterns of both genes. On the other hand,expression of p53 gene is also found to be in the state of activation in transformant, but no heavy staining of p53 protein appeared in the transformant-derived tumor with p53 specific McAb HD 200. This might be result from the partlal deletion of p53 cDNA in transformant by reverse transcriptional polymerase chain reaction(RT PCR). These results imply that activation of H-ras and c-myc genes coupled with inactivation of p53 turnor suppressor gene play important role in a particle-induced cell trans formation.