Proteomic analysis for detecting serum biomarkers related to smoking in humans

作     者：XIAO Dan ZHAO Li-juan CHU Shui-lian JING Hang WANG Chen 

作者机构：Beijing Key Laboratory of Respiratory and Pulmonary Circulation Disorders Beijing Institute of Respiratory Medicine WHO Collaborating Center for Tobacco or Health Beijing 100020China Department of Respiratory Diseases Beijing Tiantan Hospital Capital Medical University Beijing 100050 China Department of Respiratory Epidemiology Research Beijing Chao-Yang Hospital Capital Medical University Beijing 100020 China Beijing Hospital Ministry of Health Beijing 100730 China 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2012年第125卷第17期

页      面：3027-3032页

核心收录：

学科分类：10[医学] 

基　　金：supported by : Beijing Nova Program 

主　　题：smoking serum biological markers proteomics 

摘      要：Background Smoking is the leading cause of death in the world. This study focused on the difference of the serum proteomic profiling between healthy smokers and nonsmokers in order to find smoking-specific serum biomarkers. Methods Pattern-based proteomic profiling of 100 serum samples （from 50 Chinese male smokers and 50 matched nonsmokers） was performed through magnetic bead fractionation coupled with matrix-assisted laser desorption/ionization time-of-flight mass spectrometry analysis （MALDI-TOF-MS） and resulting data were statistically analyzed by Ciphergen ProteinChip software 3.0.2. Results We found 72 serum peaks were significantly different between smokers and nonsmokers （P 〈0.05）. Marker peaks of mass-to-charge ratio （m/z） 3159.13, 7561.03 and 9407.32 were smoking-specific. Conclusion The preliminary data suggested that smoking-specific serum biomarkers could be detected in humans.