Serum protein profile of yang-deficiency constitution in traditional Chinese medicine revealed by protein microarray analyses

作     者：Shuxian Sun Xiuping Zhang Jiayi Ma Cheng Ni Xiao Ying Ji Wang Lingru Li Wenlin Yuan Xujun Heng Jing Xia 

作者机构：Center for Studies in Constitution Research of Traditional Chinese MedicineBeijing University of Chinese MedicineBeijing 100029China School of Traditional Chinese MedicineTianjin University of Traditional Chinese MedicineTianjing 300193China 

出 版 物：《Journal of Traditional Chinese Medical Sciences》 (中医科学杂志（英文）)

年 卷 期：2019年第6卷第1期

页      面：67-74页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：This study was supported by the National Natural Science Foundation of China:Surface Project(81373504) National Key Basic Research Program of China(973 Program)(2011CB505400) 

主　　题：Yang-deficiency constitution Protein microarray PI3K-Akt Endocrine Energy metabolism 

摘      要：Background:Based on the theory of traditional Chinese medicine (TCM) and preepidemiological investigation,Professor Qi Wang classified the entire human population into nine constitutions and put forward the theory of Nine-Constitution Medicine. Among these constitutions,the main feature of the yang-deficiency constitution (YADC) is intolerance of the cold,which has been proven to reduce quality of life and confer susceptibility to specific *** studies explored the genetic and transcriptional bases of *** this experiment,we explored the potential mechanism of YADC using protein microarray,to deepen our understanding of its biological ***:Subjects identified with a YADC (n =12) or a balance constitution (BC;n =12) in accordance with the Classification and Determination Standards of Constitutions in Traditional Chinese Medicine were *** was collected to separate serum and protein microarray technology was used to analyze serum protein ***:The clustering of subjects constitutions based on protein expression profiling largely coincided with the TCM *** on false discovery rate correction (P .01) and fold change ≥ 5 or ≤ 0.2,a total of 85 proteins differentially expressed in YADC compared with their status in BC were selected,including 64 upregulated and 21 downregulated *** analysis suggested that subjects with YADC are susceptible to endocrine and energy metabolism disorders,as well as decline in immune ***:This study revealed that YADC exhibits systematic differences in its protein expression ***,we can potentially explain the characteristics of YADC partly via differentially expressed proteins.