Effects of Copper-phenanthroline on Pentachlorophenol-induced Adaptation and Cell Death of Escherichia coli

作     者：XUE-WEN ZHANG RONG-GUI LI XIN WANG AND SHUAN-HU ZHOU 

作者机构：Department of Biology Qingdao University Qingdao 266071 Shandong China Department of Neurosurgery Department of Orthopedic Surgery Brigham and Women＇s Hospital Harvard Untverstty Medical School Boston MA 02115 USA 

出 版 物：《Biomedical and Environmental Sciences》 (生物医学与环境科学（英文版）)

年 卷 期：2007年第20卷第2期

页      面：106-112页

核心收录：

学科分类：100405[医学-卫生毒理学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 10[医学] 

基　　金：This study was supported by The Lady David Foundation (To S. Z.) NIH/NINDS KO1NS055072 (To X. W.) Natural Science Foundation of Shandong Province, China (No.2006ZRB01451) (To R. L.) 

主　　题：Adaptation Cell death Cytotoxicity Escherichia coli Oxidative phosphorylation Pentachlorophenol 1, 10-phenanthroline-copper 

摘      要：Objective To evaluate the effects of copper-phenanthroline （CuOP） on pentachlomphenol （PCP）-induced adaptation and cell death of Escherichia coli. Methods Bacterial growth and adaptation to PCP were monitored spectrophotometrically at 600 nm. Inactivation of bacterial cells was determined from colony count on agar dishes. Cellular ATP content and accumulation of PCP were assessed by chemiluminescence and HPLC analysis respectively. The formation of PCP-Cu-OP complex was shown by UV-visible spectra. Results Escherichia coli （E. coli） could adapt to PCP, a wood preservative and insecticide used in agriculture. The adaptation of E. coli to PCP prevented its death to the synergistic cytotoxicity of CuOP plus PCP and declined cellular accumulation and uncoupling of oxidative phosphorylation of PCP. Furthermore, CuOP and PCP neither produced reactive oxygen species （ROS） nor had a synergistic effect on uncoupling of oxidative phosphorylation in E. coli. The synergistic cytotoxicity of CuOP and PCP in E. coli might be due to the formation of lipophilic PCP-Cu-OP complex. Conclusion Our data suggested that adaptation of E. coli to PCP decreased the synergistic effects of CuOP and PCP on prokaryotic cell death due to the formation of lipophilic PCP-Cu-OP complex, but it had no effect on the uncoupling of oxidative phosphorylation and production of reactive oxygen species in E. coli.