新型内切肝素酶酶解产物肝素二糖的液相色谱分离和电喷雾质谱表征
Separation and Characterization of Heparin Disaccharides Digested by A Novel Endolytic Heparinase Using High Performance Liquid Chromatography and Electrospray Mass Spectrometry作者机构：中国科学院化学研究所，北京质谱中心，北京100080 中国科学院微生物研究所，北京100080
出 版 物：《质谱学报》 (Journal of Chinese Mass Spectrometry Society)
年 卷 期：2004年第25卷第B10期
页 面：33-34页
核心收录：
学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 070303[理学-有机化学] 0703[理学-化学]
基 金：国家自然科学基金(20035010、20175033)
主 题：内切肝素酶 酶解产物 肝素二糖 液相色谱 分离 电喷雾质谱
摘 要：Five major disaccharide peaks were obtained from the digests of heparin by a novel heparinase from the species of Sphingobacterium on the high performance liquid chromatography (HPLC). The electrospray ionization-mass spectrometry of peak I to V sequentially represented one nonsulfated disaccharide, two monosulfated disaccharides,two disulfated disaccharides. No trisulfated disaccharide was detected. The findings revealed that the heparinase specifically eliminated undersulfated domain in heparin.
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