A nano-functionalized real-time electrochemiluminescent biosensor for alanine transaminase assay

作     者：CHU HaiHong WU MeiSheng CAI Xia TU YiFeng 

作者机构：Institute of Analytical ChemistryDepartment of ChemistrySoochow UniversitySuzhou 215123China 

出 版 物：《Science China Chemistry》 (中国科学（化学英文版）)

年 卷 期：2011年第54卷第5期

页      面：816-821页

核心收录：

学科分类：081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 070302[理学-分析化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 0703[理学-化学] 

基　　金：supported by the National Natural Science Foundation of China (20275025 & 20675055) the Natural Science Fundation of Jiangsu Province (BK2009111) Technology Plan of Suzhou (SYJG0901) 

主　　题：alanine aminotransferase electrochemiluminescent biosensor luminol pyruvate oxidase carbon nanotubes 

摘      要：An electrochemiluminescent (ECL) biosensor was constructed for selective assay of alanine aminotransferase (ALT) based on the enzymatically catalyzed oxidation of pyruvate by pyruvate oxidase (PYOD). The composite of potassium ferricyanide and carbon nanotube was adopted to pre-functionalize the basal platinum electrode while the potassium ferricyanide acted as the activator of PYOD. The ALT catalyzed the reaction of L-alanine and-ketoglutarate to produce pyruvate which could be further enzymatically oxidized by PYOD to yield H2O2 to intensify the ECL of luminol. The biosensor showed rapid response for real-time measurement of ALT in the linear concentration range from 0.00475 to 350 U/L (r = 0.993) with a relatively standard deviation of 2.5% (CALT = 47.5 U/L,n = 6). The biosensor was applied to assay the ALT in rat serum with average recovery of 90.5%.