Microsatellite-Aided Screening for Fertility Restoration Genes (Rf) Facilitates Hybrid Improvement

作     者：Raafat El-Namaky Saber Sedeek Yonnelle Dea Moukoumbi Rodomiro Ortiz Baboucarr Manneh 

作者机构：Africa Rice Center (Africa Rice) Sahel Regional Station B.P.96 Saint-Lauis Senegal Africa Rice Center (Africa Rice) P.O.Box 33581 Dar es Salaam Tanzania Swedish University of Agricultural Sciences Box 101 Alnarp SE 23053 Sweden Rice Research and Training Center (RRTC) Sakha-33717 Egypt 

出 版 物：《Rice science》 (水稻科学（英文版）)

年 卷 期：2016年第23卷第3期

页      面：160-164页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 0902[农学-园艺学] 

主　　题：cytoplasmic male sterility fertility restoration gene heterosis rice simple sequence repeat 

摘      要：DNA markers enabled to determine the chromosomal locations of the two Rf genes(Rf3 and Rf4) in the wild-abortive cytoplasmic male sterility(WA-CMS) system. Four simple sequence repeats(SSRs) RM171, RM258, RM315 and RM443 were used to detect the allelic status with respect to the fertility restoration genes(Rf3 and Rf4) in 300 rice cultivars or breeding lines. The results revealed that out of 300 lines, 90 lines screened had Rf3, 65 lines had Rf4, and 45 lines had Rf3 and Rf4 alleles. Furthermore, 45 lines selected using SSR markers were mated with a CMS line(IR58025A) to analyze their restoring ability. Offspring of all the test lines except HHZ8-SAL9DT1-Y1, HHZ5-SAL9-Y3-1 and IDSA77 exhibited higher pollen and spikelet fertility( 80%), thus confirming they bear the Rf alleles. The hybrid offspring of ARH12-6-1-1-B-3-1, IR32307-10-3-2-1 and Sahel 329 had the highest pollen fertility(97.39%, 98.30% and 97.10%, respectively) and spikelet fertility(95.10%, 97.07% and 96.10%, respectively).