微管蛋白的聚合和变性及紫杉醇的影响

作     者：杨勇 王保怀 李芝芬 张有民 韩公社 阎隆飞 

作者机构：北京大学物理化学研究所北京100871 北京农业大学生物学院北京100094 

出 版 物：《物理化学学报》 (Acta Physico-Chimica Sinica)

年 卷 期：1999年第15卷第2期

页      面：182-185页

核心收录：

学科分类：0710[理学-生物学] 1007[医学-药学(可授医学、理学学位)] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 10[医学] 

基　　金：国家自然科学基金!29573090 

主　　题：微管蛋白 紫杉醇 DSC 聚合 变性 

摘      要：The thermal properties of porcine brain tubulin polymerization and denaturation and the efftcts of taxol were studied from 4 ℃ to 115 ℃ with a new highly-sensitive differential scanming calorimeter (Micro DSC Ⅲ, Setaram,France). The DSC results indicated that microtubule polymerization can proceed uia an endothermic process and that taxol could alter the process.A large aggregation peak was found at about 85 ℃ in the course of the denaturation and it disap pcared with adding taxol. The heat flow oscillation was observed during the controlled-temperature experiments and taxol could stabilize the structure of the microtubules so that the oscillation disappezred.