Reliable experimental model of hepatic veno-occlusive disease caused by monocrotaline

作     者：Miao-Yan Chen Jian-Ting Cai Qin Du Liang-Jing Wang Jia-Min Chen Li-Ming Shao 

作者机构：Department of Gastroenterology and Hepatology Second Affiliated Hospital Zhejiang University School of Medicine Hangzhou 310009 China. 

出 版 物：《Hepatobiliary & Pancreatic Diseases International》 (国际肝胆胰疾病杂志（英文版）)

年 卷 期：2008年第7卷第4期

页      面：395-400页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：hepatic veno-occlusive disease monocrotaline proliferating cell nuclear antigen 

摘      要：BACKGROUND:Hepatic veno-occlusive disease(HVOD)is a severe complication of chemotherapy before hematopoietic stem cell transplantation and dietary ingestion of pyrrolizidine *** experimental models were established to study its mechanisms or therapy,but few are *** work aimed at evaluating a rat model of HVOD induced by monocrotaline to help advance research into this disease. METHODS:Thirty-two male rats were randomly classified into 5 groups,and PBS or monocrotaline was administered (100 mg/kg or 160 mg/kg).They were sacrificed on day 7(groups A,B and D)or day 10(groups C and E).Blood samples were collected to determine liver enzyme *** weight of the liver and body and the amount of ascites were *** changes of liver tissue on light microscopy were assessed by a modified Deleve scoring *** positivity of proliferating cell nuclear antigen(PCNA)was estimated. RESULTS:The rats that were treated with 160 mg/kg monocrotaline presented with severe clinical symptoms (including two deaths)and the histopathological picture of *** the other hand,the rats that were fed with 100 mg/kg monocrotaline had milder and reversible *** of the rats sacrificed on day 10 with those sacrificed on day 7 showed that the positivity of PCNA increased,especially that of ***:Monocrotaline induces acute,dose- dependent HVOD in *** model is potentially reversible with a low dose,but reliable and irreversible with a higher *** modified scoring system seems to be more accurate than the traditional one in reflecting the histopathology of *** enhancement of PCNA positivity may be associated with hepatic tissue undergoing recovery.