Effects of propofol on ammonium chloride-exposed astrocyte morphology and aquaporin-4 expression

作     者：Hanjian Chen Caifei Pan Peng Guo Yueying Zheng Shengmei Zhu 

作者机构：Department of Anesthesiology First Affiliated Hospital Zhejiang University Medical School Hangzhou 310003 Zhejiang Province China Department of Anesthesiology Shaoxing Central Hospital Shaoxing 312030 Zhejiang Province China 

出 版 物：《Neural Regeneration Research》 (中国神经再生研究（英文版）)

年 卷 期：2011年第6卷第5期

页      面：383-387页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 1002[医学-临床医学] 081704[工学-应用化学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 08[工学] 0817[工学-化学工程与技术] 071006[理学-神经生物学] 

基　　金：Supported by a Grant from Zhejiang Provincial Health Department  No. 2007A057 

主　　题：ammonium chloride aquaporin-4 astrocytes propofol protein kinase C 

摘      要：Ammonia induces astrocyte swelling, which is strongly associated with overexpression of aquaporin-4. However, the mechanisms by which ammonia induces astrocyte swelling, and subsequently upregulating aquaporin-4 expression, remain unknown. In the present study, astrocytes were cultured in vitro and exposed to ammonium chloride （NH4CI）, followed by propofol protein kinase C agonist, or antagonist, respectively. Astrocyte morphology was observed by light microscopy, and aquaporin-4 expression was detected by western blot analysis. Results showed that propofol or protein kinase C agonist significantly attenuated the degree of NH4CI-induced astrocyte swelling and inhibited increased aquaporin-4 expression. Propofol treatment inhibited aquaporin-4 overexpression in cultured astrocyte induced by NH4CI; protein kinase C pathway activation is potentially involved.