Effects of di-n-butyl phthalate on male rat reproduction following pubertal exposure
Effects of di-n-butyl phthalate on male rat reproduction following pubertal exposure作者机构：State key Laboratory of Reproductive Medicine Nanjing Medical University Nanjing 210029 China Key Laboratory of Reproductive Medicine Institute of Toxicology Nanjing Medical University Nanjing 210029 China Jiangsu Provincial Center for Disease Control and Prevention Nanjing 210009 China
出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)
年 卷 期：2011年第13卷第5期
页 面：702-709页
核心收录：
学科分类：0710[理学-生物学] 081702[工学-化学工艺] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学]
基 金：ACKNOWLEDGMENTS We gratefully acknowledge the assistance of Feng Chen with the statistical analysis. The study was supported by grants from the National Basic Research Program of China (973 Program) (No. 2009CB941703) Jiangsu Provincial Natural Science Funds (No. BK2007235) and the National Natural Science Foundation of China (No. 30901222)
主 题：di-n-butyl phthalate hormones proteomics spermatogenesis testis
摘 要：Di-n-butyl phthalate （DBP） is an endocrine-disrupting chemical that has the potential to affect male reproduction. However, the reproductive effects of low-dose DBP are still not well known, especially at the molecular level. In the present study, pubertal male Sprague-Dawley rats were orally administered DBP at a wide range of doses （0.1, 1.0, 10, 100 and 500 mg kg^-1 day^-1） for 30 days. The selected end points included reproductive organ weights, testicular histopathology and serum hormonal levels. Additionally, proteomic analysis was performed to identify proteins that are differentially expressed as a result of exposure to DBP at low doses （0.1, 1.0 and 10 mg kg^-1 day^-1）. Toxic effects were observed in the high-dose groups, including anomalous development of testes and epididymides, severe atrophy of seminiferous tubules, loss of spermatogenesis and abnormal levels of serum hormones. Treatment with low doses of DBP seemed to exert a ＇stimulative effect＇ on the serum hormones. Proteomics analysis of rat testes showed 20 differentially expressed proteins. Among these proteins, alterations in the expression of HnRNPA2/B1, vimentin and superoxide dismutase 1 （SOD1） were further confirmed by Western blot and immunohistochemistry. Taken together, we conclude that high doses of DBP led to testicular toxicity, and low doses of DBP led to changes in the expression of proteins involved in spermatogenesis as well as changes in the number and function of Sertoli and Leydig cells, although no obvious morphological changes appeared. The identification of these differentially expressed proteins provides important information about the mechanisms underlying the effects of DBP on male rat reproduction.
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