Polymorphisms of the oxidant enzymes glutathione S-transferase and glutathione reductase and their association with resistance of Plasmodium falciparum isolates to antimalarial drugs

作     者：Raewadee Wisedpanichkij Wanna Chaicharoenkul Poonuch Mahamad Prapichaya Prompradit Kesara Na-Bangchang 

作者机构：Pharmacology and Toxicology UnitGraduate Program in Biomedical SciencesThammasat UniversityThailand 

出 版 物：《Asian Pacific Journal of Tropical Medicine》 (亚太热带医药杂志（英文版）)

年 卷 期：2010年第3卷第9期

页      面：673-677页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by Thammasat University and The Commission on Higher Education Ministry of Education of Thailand 

主　　题：Plasmodium falciparum Drug resistance Glutathione reductase(PfGR) Glutathione S-transferase(P/GST) 

摘      要：Objective:To investigate the association between amplification of the two regulatory genes controlling glutathione(GSH) levels,glutathione reductase(PfGR) and glutathione S-transferase (PfGST) genes and sensitivity of Plasmodium falciparum(***) isolates collected from different malaria endemic areas of Thailand to standard antimalarial ***:A total of 70 *** isolates were collected from endemic areas of multi-drug resistance (Tak,Chantaburi and Ranong Provinces) during the year *** in vitro assessment of antimalarial activity of *** clones(K1- and Dd2 chloroquine resistant and 3D7- chloroquine sensitive) and isolates to chloroquine,quinine,mefloquine and arteusnate was performed based on SYBR Green modified ***:68(97.14%),11(15.71%) and 28(40%) isolates respectively were classified as chloroquine-,quinine- and mefloquine-resistant isolates. With this limited number of *** isolates included in the analysis,no significant association between amplification of PfGST gene and sensitivity of the parasite to chloroquine, quinine,mefloquine and quinine was *** on PCR analysis,Dd2,Kl and 3D7 clones all contained only one copy of the PfGST *** isolates(70) also carried only one copy number of PfGST *** appears to be an association between amplification of PfGR gene and chloroquine *** 3D7 and Dd2 clones were found to carry only one PfGR gene copy, whereas the K1 clone carried two gene ***:Chloroquine resistance is likely to be a consequence of multi-factors and enzymes in the GSH system may be partly involved. Larger number of parasite isolates are required to increase power of the hypothesis testing in order to confirm the involvement of both genes as well as other genes implicated in glutathione metabolism in conferring chloroquine resistance.