反胶团相转移法提纯酵母脂肪酶

作     者：褚莹 纪建业 马占芳 于艳春 吴子生 

作者机构：东北师范大学化学系长春130024 通化师范学院化学系通化134000 

出 版 物：《高等学校化学学报》 (Chemical Journal of Chinese Universities)

年 卷 期：1997年第18卷第10期

页      面：1695-1697页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：国家自然科学基金 

主　　题：反胶团 相转移 酵母脂肪酶 脱氧胆酸 提纯 

摘      要：The preliminary result of the yeast-lipase purified and separated by the reversemicelles system of AOT/iso-octane is reported in the paper. The phase transfer mechanismof the static interaction in the process of the phase transfer of reverse micelles as well as thehydroplic interaction was studied based on the extractive efficiency of lipase and the recoveryefficiency of protein affected by pH value of water phase and salt concentration. Theinteraction between enzyme molecules and reverse micelles was reduced and the recovery efficiencyof protein and enzyme activity was increased significantly when deoxycholic acid was addedinto the reverse micelles of AOT/iso-octane as the biological surfactant. The relative activityof lipase recovered was increased by 4%~9% compared with origin enzyme aqueous in therange of pH investigated.