Ursolic Acid Prevents Retinoic Acid-Induced Bone Loss in Rats

作     者：CHENG Min LIANG Xu-hua WANG Qing-wei DENG Ya-ting ZHAO Zhi-xin LIU Xue-ying 

作者机构：College of Biology Pharmacy and Food Engineering Shangluo University Department of Pharmacy The Second Affiliated Hospital Fourth Military Medical University Department of Pharmacology Xi'an Medical College Department of Medicinal Chemistry Fourth Military Medical University 

出 版 物：《Chinese Journal of Integrative Medicine》 (中国结合医学杂志（英文版）)

年 卷 期：2019年第25卷第3期

页      面：210-215页

核心收录：

学科分类：10[医学] 

基　　金：Supported by Qinling-Bashan Mountains Bioresources Comprehensive Development Collaborative Innovation Center(No.QBXT-Z(Z)-15-4) 

主　　题：osteoporosis bone turnover ursolic acid bone mineral density biomechanics microcomputed tomography 

摘      要：Objective: To examine the effects of ursolic acid(UA) on mitigating retinoic acid(RA)-induced osteoporosis in rats. Methods: Fifty female Sprague-Dawley rats were randomly divided into the control group(n=10) and the osteoporosis group(n=40). The 40 osteoporosis rats were induced by 75 mg/(kg·d) RA once daily for 2 weeks, and then were randomly assigned to vehicle control(model), low-, middle-, and high-dose UA [(UA-L, UA-M, UA-H; 30, 60, 120 mg/(kg·d), respectively] groups(10 rats each). UA were administered once daily to the rats from the 3 rd weeks for up to 4 weeks by gavage. Bone turnover markers [serum alkaline phosphatase(ALP), osteocalcin(OCN), urine deoxypyridinoline(DPD)] and other parameters, including serum calcium(S-Ca), serum phosphorus(S-P), urine calcium(U-Ca), urine phosphorus(U-P), and bone mineral density(BMD) of the femur, 4 th lumbar vertebra and tibia, bone biomechanical properties and trabecular microarchitecture, were measured. Results: The osteoporosis in rats was successfully induced by RA. Compared with the model group, UA-M and UA-H significantly reversed the RA-induced changes in S-P, U-Ca, U-P, ALP, OCN and urine DPD ratio and markedly enhanced the BMD of right femur, 4 th lumbar vertebra and tibia(P0.05 or P0.01). Further, biomechanical test and microcomputed tomography evaluation also showed that UA-H drastically improved biomechanical properties and trabecular microarchitecture(P0.05 or P0.01). Conclusion: UA could promote bone formation, increase osteoblastic activity and reduce osteoclastic activity in rats, indicating that UA might be a potential therapeutic of RA-induced acute osteoporosis.