Investigation of the Holding Capability of the Dielectrophoretic Gate and Sorter System for Biodetection

作     者：Hongjun Song Ziliang Cai Dawn J. Bennett 

作者机构：CFD Research Corporation Huntsville USA Department of Mechanical Engineering Louisiana State University Baton Rouge USA Science Mathematics and Technology Division Burlington County Community College 500 College Circle Mt Laurel USA 

出 版 物：《Open Journal of Applied Biosensor》 (生物传感器（英文）)

年 卷 期：2013年第2卷第2期

页      面：65-75页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Biodetection Dielectrophoresis Microfludic System Particle Separation 

摘      要：The dielectrophoretic gate and sorter system has been widely applied for preconcentrating and sorting of bioparticles for biodetection. In such systems, the dielectrophoretic force is generated by applying an AC electric field on the three dimensional electrode systems (containing a pair of electrodes on the top and bottom of the channel). Particles are held and sorted by balancing the DEP force with the hydrodynamic drag force. The holding capability is very important for such systems because it determines the preconcentration and sorting efficiency. In this paper, we investigate the holding capability of a simple dielectrophoretic gate system. Initially, a three dimensional numerical scheme was introduced to estimate the holding capability and was further validated by comparing with experimental results. Second, we systematically investigated the effects of the phase difference between the top and bottom electrodes;the height and width of the channel, and the relative position and size of top and bottom electrodes. The results demonstrated that the maximum holding capability is reached when the phase difference between the top and bottom electrodes is around 180o. The results also show that the holding capability varied with the size and relative position of electrodes on the top and bottom, and the maximum holding capability is obtained when the top and bottom electrodes had the same size and the centers of both electrodes overlapped.