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Research on critical technology of micro/nano bioparticles manipulation platform based on light-induced dielectrophoresis

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Science China(Technological Sciences) 2009年第10期52卷 2831-2839页

作者： NI ZhongHua YI Hong ZHU ShuCun SONG ChunFeng Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments School of Mechanical EngineeringSoutheast UniversityNanjing 210096China

On the basis of the research on the status and problems of micro/nano bio-particles manipulation using dielectrophoresis, the theoretical basis and the model simulation of micro/nano bio-particles manipu-lation using light-induced dielectrophoresis were discussed. The space distribution of electric field and dielectrophoresis forces in different heights were also obtained. On this basis, the core component of the micro manipulation system, that is, photoconductive layer of the chip, was completed in the mate-rial selection, fabricating process and performance analysis testing. Then the voltage drop of the sus-pension and the effective voltage frequency spectrum were obtained. Finally, by combining the machine vision detection with real-time tracking system, the micro/nano bio-particles manipulation platform based on the light-induced dielectrophoreisis was established, and then the manipulations for micro/nano bio-particles, such as quick collection, transport, separation, were implemented. This provided a basis for rapid, accurate, and low-cost detection of serious diseases based on the micro-fluidic biochip and early diagnosis.

关键词： light-induced dielectrophoresis micro-fluidic biochip dielectric polarization mechanism separation chip

Effective removal of Cd^2+ and Pb^2+ pollutants from wastewater by dielectrophoresis-assisted adsorption

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Frontiers of Environmental Science & Engineering 2019年第2期13卷 21-27页

作者： Qinghao Jin Chenyang Cui Huiying Chen Jing Wu Jing Hu Xuan Xing Junfeng Geng Yanhong Wu College of Life and Environmental Science Minzu University of ChinaBeijing 100081China Beijing Engineering Research Center of Food Environment and Public Health Minzu University of ChinaBeijing 100081China Institute for Materials Research and Innovation Institute for Renewable Energy and Environmental TechnologiesUniversity of BoltonBL3 5AB BoltonUK

dielectrophoresis(DEP)was combined with adsorption(ADS)to simultaneously and effectively remove Cd^2+ and Pb^2+ species from aqueous *** implement the process,bentonite particles of submicro-meter size were used to first adsorb the heavy metal *** particles were subsequently trapped and removed by *** effects of the adsorbent dosage,DEP cell voltage and the capture pool numbers on the removal rate were investigated in batch processes,which allowed us to determine the optimal experimental *** high removal efficiency,97.3% and 99.9% for Cd^2+ and Pb^2+,respectively,were achieved when the ions are coexisting in the *** microstructure of bentonite particles before and after ADS/DEP was examined by scanning electron *** results suggest that the dielectrophoresis-assisted adsorption method has a high capability to remove the heavy metals from wastewater.

关键词： Adsorption dielectrophoresis Heavy metals Cadmium Lead Wastewater

Microwire formation based on dielectrophoresis of electroless gold plated polystyrene microspheres

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Chinese Physics B 2011年第5期20卷 401-408页

作者：姜洪源任玉坤陶冶 School of Mechatronics Engineering Harbin Institute of Technology State Key Laboratory of Fluid Power Transmission and Control Zhejiang University

Microspheres coated with a perfectly conductive surface have many advantages in the applications of biosensors and micro-electromechanical systems. Polystyrene microspheres with the diameter of 10 μm were coated with a 50 nmthick gold layer using an electroless gold plating approach. dielectrophoresis （DEP） for bare microspheres and shelled microspheres was theoretically analysed and the real part of the Clausius Mossotti factor was calculated for the two kinds of microspheres. The experiments on the dielectrophoretic characterisation of the uncoated polystyrene microspheres and gold coated polystyrene microspheres （GCPMs） were carried out. Experimental results showed that the gold coated polystyrene microspheres were only acted by a positive dielectrophoretic force when the frequency was below 40M Hz, while the uncoated polystyrene microspheres were governed by a negative dielectrophoretic force in this frequency range. The gold coated polystyrene microspheres were exploited to form the rnicrowire automatically according to their stable dielectrophoretic and electric characterisations.

关键词： dielectrophoresis gold coated polystyrene microspheres microwire

Effects of Chip Geometries on dielectrophoresis and Electrorotation Investigation

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Chinese Journal of Mechanical Engineering 2014年第1期27卷 103-110页

作者： REN Yukun WU Hongchi FENG Guojing HOU Likai JIANG Hongyuan School of Mechatronics Engineering Harbin Institute of Technology State Key Laboratory of Fluid Power Transmission and Control Zhejiang University The First Affiliated Hospital Harbin Medical University

The electric fields employed for such work are generated using chips, such as planar linear interdigitated arrays or two parallel arrays. However, chip geometries usually affect the investigation of dielectrophoresis （DEP） and electrorotation （ER） significantly, and even may misdirect the theoretical prediction. In order to understand the electrodes geometries effect and provide a suitable range of parameters, three-dimensional simulations for the DEP and ER characterizations on the quadrupolar hyperbolical electrodes are carried out. Influences of the electrodes gaps, cell height, work region, energized voltage and frequencies for the DEP and ER manipulations are analyzed, and the analysis results show that the gaps of the electrodes and the cell height have enormous effects, but the work region is not so important. Moreover, depending on the theoretical analysis, ER experiments for polystyrene microspheres with the diameter of 20 ~m are carried out on two kinds of chips. The experimental results show that the microspheres rotate in the counter-field direction and the maximum rotation speed appears in the megahertz range. In addition, the experimental results are compared with the simulation results, showing that the three-dimensional simulations considering the chip geometries are more accurate than the two-dimensional predictions. This paper provides a new understanding for the theoretical predictions of DEP and ER manipulations, which decreases the difference of the theoretical and experimental results significantly, and will be significant for the lab chip research.

关键词： dielectrophoresis electrorotation chip geometries

Principles of carbon nanotube dielectrophoresis

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Nano Research 2021年第7期14卷 2188-2206页

作者： Wenshan Li Frank Hennrich Benjamin S.Flavel Simone Dehm Manfred Kappes Ralph Krupke Institute of Nanotechnology Karlsruhe Institute of TechnologyKarlsruhe 76021Germany Institute of Physical Chemistry Karlsruhe Institute of TechnologyKarlsruhe 76021Germany Institute of Materials Science Technische Universität DarmstadtDarmstadt 64287Germany Institute for Quantum Materials and Technologies Karlsruhe Institute of TechnologyKarlsruhe 76021Germany School of Mechanical Engineering Shanghai Jiao Tong UniversityNo.800 Dongchuan RoadShanghai 200240Chin

dielectrophoresis(DEP)describes the motion of suspended objects when exposed to an inhomogeneous electric *** has been successful as a method for parallel and site-selective assembling of nanotubes from a dispersion into a sophisticated device *** have conducted extensive works to understand the DEP of nanotubes in aqueous ionic surfactant ***,only recently,DEP was applied to polymer-wrapped single-walled carbon nanotubes(SWCNTs)in organic solvents due to the availability of ultra-pure SWCNT *** this paper,the focus is on the difference between the DEP in aqueous and organic *** starts with an introduction into the DEP of carbon nanotubes(CNT-DEP)to provide a comprehensive,in-depth theoretical background before discussing in detail the experimental procedures and *** academic interests,this work focuses on the CNT-DEP deposition scheme,discusses the importance of the electrical double layer,and employs finite element simulations to optimize CNT-DEP deposition condition with respect to the experimental *** important outcome is an understanding of why DEP in organic solvents allows for the deposition and alignment of SWCNTs in low-frequency and even static electric fields,and why the response of semiconducting SWCNTs(s-SWCNTs)is strongly enhanced in non-conducting,weakly polarizable *** to further improve CNT-DEP for s-SWCNT-relevant applications are given as ***,this work should serve as a practical guideline to select the appropriate setting for effective CNT DEPs.

关键词： single-walled carbon nanotubes hydrodynamics assembly deposition alignment polarizability dielectrophoresis

Experimental study on filtering,transporting, concentrating and focusing of microparticles based on optically induced dielectrophoresis

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Science China(Technological Sciences) 2010年第9期53卷 2388-2396页

作者： ZHU XiaoLu,YIN ZhiFeng,GAO ZhiQiang & NI ZhongHua Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments,Southeast University,Nanjing 211189,China Jiangsu Key Laboratory for Design and Manufacture of Micro-Nano Biomedical Instruments Southeast University Nanjing China

The key problem to be solved for the dielectrophoresis (DEP) application is to provide dynamically reconfigurable microelectrodes and low-cost methodology for bioparticle *** emergence of optically induced DEP (ODEP) based on photoconductive effect provides a potential solution for the above *** this paper,an ODEP chip is designed and fabricated,and the corresponding experimental platform was established,whereupon four types of particle manipulation regimes–filtering,transporting,concentrating and focusing based on ODEP are experimentally demonstrated and the operating performances are quantitatively *** experiment results show that the functions and performances of ODEP manipulation are heavily dependent on the geometrical shape,scales and speed of optical patterns,actuating signal frequency and the electric conductivity of the *** manipulation efficiency can increase by more than 50% via increasing the optical line ***,the efficiency is obviously affected by the inclination angle of the optical oblique lines in the manipulation of particle ***,the maximum velocity of particles increases with the increment of the inside radius and the thickness of the optical trapping *** manipulation efficiency is always related to signal frequency and solution conductivity,and empirically,satisfactory performance and high efficiency are obtained when the solution electric conductivity ranges from 5×10-4 S/m to 5×10-3 S/m.

关键词： optically-induced dielectrophoresis particle manipulation filtering transporting concentrating focusing

Separation of nanocolloids driven by dielectrophoresis:A molecular dynamics simulation

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Science China(Technological Sciences) 2009年第7期52卷 1874-1881页

作者： NI ZhongHua ZHANG XinJie YI Hong Jiangsu Key Laboratory for Design and Manufacture of Micro/Nano Biomedical Instruments School of Mechanical EngineeringSoutheast UniversityNanjing 210096China

The nonequilibrium molecular dynamics (MD) method was used to model the nanocolloids and the solvent particles. By introducing a non-uniform electric field, colloids were polarized to have opposite polarities. Separation of colloids driven by dielectrophoresis (DEP) could be seen clearly under a strong electric field at low temperatures. Analyzing the ratio of DEP velocities of colloids to thermal velocities of neutral solvent particles showed that when the ratio was correspondingly big, collision between colloids and solvent particles would be intense, making the DEP velocity of colloids fluctuate frequently. By changing the electric field strength, it was found that the enhancement of electric field strength would quicken the separation of colloids. But when the electric field strength increased to a certain degree, the separation motion would be slow because of the strong friction resistance of the solvent particles to the colloids. Moreover, studying the separation reason of colloids based on the potential energy showed that after colloids were polarized, the attractive potential energy among the colloids would be weaker than before, while the increase of temperature would reduce the attractive potential energy and increase the repulsive potential energy, which accorded with the DLVO theory.

关键词： molecular dynamics dielectrophoresis DLVO theory

Manipulation and Separation of Particles of Metal Oxides by dielectrophoresis

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Chemical Research in Chinese Universities 2010年第4期26卷 645-648页

作者： CHEN Hui-ying ZHU Yue-lin LIU Yan ZHANG He-teng YU Le LI Di School of Material Science and Engineering Beihang University Beijing 100083 P. R. China College of Life and Environmental Science Minzu University of China Beijing 100081 P. R. China

With the development of nanotecbnology, the separation and manipulation of micro-nano-panicles have become a research focus in the field of nano-materials, nielectrophoresis（DEP） is a non-contact technology for the separation and manipulation of micro-nano-particles. Here is reported the design and fabrication of a DEP based microchip with microelectrode arrays for capturing micro-particles of inorganic oxides in petroleum. The DEP behavior of micro-particles of inorganic oxides in oil media was explored via this microchip. The microchip shows relatively a good DEP response to inorganic oxides in oil media. Furthermore, much more factors were explored such as fiequency（Hz）, and particle size（μm）, as well as metal valence. As a conclusion, the best frequency is 50 Hz. It is expected to capture panicles with different sizes or separate different oxide panicles by regulating DEP conditions. Thus, a new method could be established for the separation and purification panicles of different oxides, as well as the separation and manipulation of an oxide with different particle sizes.

关键词： dielectrophoresis Microchip Inorganic metal oxide Separation

Improvement Design of Biochip Towards High Stable Bioparticle Detection Utilizing dielectrophoresis Impedance Measurement

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Transactions of Nanjing University of Aeronautics and Astronautics 2015年第2期32卷 143-147页

作者：黄海波钱成李相鹏陈立国徐文奎郑亮孙立宁 Robotics and Microsystems Center College of Mechanical and Electrical Engineering & Collaborative Innovation Center of Suzhou Nano Science and TechnologySoochow University

dielectrophoresis impedance measurement(DEPIM)is a powerful tool for bioparticle detection due to its advantages of high efficiency,label-free and low ***,the strong electric field may decrease the viability of the bioparticle,thus leading to instability of impedance measurement.A new design of biochip is presented with high stable bioparticle detection capabilities by using both negative dielectrophoresis(nDEP)and traveling wave dielectrophoresis(twDEP).In the biochip,a spiral electrode is arranged on the top of channel,while a detector is arranged on the bottom of the *** influence factors on the DEP force and twDEP force are investigated by using the basic principle of DEP,based on which,the relationship between Clausius-Mossotti(CM)factor and the frequency of electric field is *** two-dimensional model of the biochip is built by using Comsol *** potential distribution,force distribution and particle trajectory in the channel are then obtained by using the simulation ***,both the simulations and experiments are performed to demonstrate that the new biochip can enhance the detection efficiency and reduce the negative effects of electric field on the bioparticles.

关键词： dielectrophoresis impedance measurement detection biosensor