Role of Cell Surface Structures in Biofilm Formation by <i>Escherichia coli</i>

作     者：Hafida Zahir Hamadi Fatima Lekchiri Souad Mliji El Mostafa Ellouali Mostafa Latrache Hassan 

作者机构：Bioprocess and Biointerface Laboratory Faculty of Science and Techniques Sultan Moulay Slimane University Beni Mellal Morocco Laboratory of Biotechnology and Valorisation of Natural Resources Department of Biology Faculty of Science University Ibn Zohr Agadir Morocco Food Safety & Environmental Department Institute Pasteur Casablanca Morocco 

出 版 物：《Food and Nutrition Sciences》 (食品与营养科学（英文）)

年 卷 期：2015年第6卷第12期

页      面：1160-1165页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Biofilm Escherichia coli Cell Surface Serotype Antigen Bacterial Adhesion 

摘      要：This study aims to understand the relationship between capabilities of Escherichia coli strains to form biofilm and serotype groups expressed on cell surface. Sixteen strains of E. coli were originally isolated from different food processing lines in different Moroccan cities. Strains serotyped based on their O (somatic), H (flagellar), and K (capsular) surface antigen profiles using different antiserums. Biofilm assays carried out in 96-well microtiter dishes using the method of O’Toole et al. Our results show that no clear relation observed between origin and serotype groups. In the other hand, we observed that not all studied strains were able to form biofilm. Furthermore, combination of antigens H40 and K11 appears to be involved in biofilm formation. In fact, the H antigen seems to be implicated in the placement of the bacterial cells near the surface and the K antigen may play a role in physicochemical interactions between bacteria and inert surface.