Detection of Epstein-Barr Virus in Nasopharyngeal Carcinoma in Sudanese by <i>in Situ</i>Hybridization

作     者：Ameera A. M. Adam Nazik E. Abdullah Lamyaa A. M. El Hassan Elwaleed M. Elamin Muntaser E. Ibrahim Ahmed M. El Hassan 

作者机构：Department of Molecular Biology Institute of Endemic Diseases University of Khartoum Khartoum Sudan Department of Surgery Faculty of Medicine University of Khartoum Khartoum Sudan Faculty of Medical Laboratory Sciences Alzaeim Alazhari University Khartoum Sudan School of Medicine Ahfad University for Women Omdurman Higher Specialization in Histopathology Khartoum Sudan 

出 版 物：《Journal of Cancer Therapy》 (癌症治疗（英文）)

年 卷 期：2014年第5卷第6期

页      面：517-522页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：EBV NPC Sudan ISH EBER 

摘      要：Objectives: The aim of this study is to detect Epstein-Barr virus (EBV) in nasopharyngeal carcinoma (NPC) biopsies of Sudanese patients using EBV-encoded RNA (EBER) in Situ hybridization (EBER-ISH). Study Design: This is a descriptive cross-sectional study conducted at the National Center for ENT diseases and Head and Neck Surgery and the Institute of Endemic Diseases, University of Khartoum, Khartoum City, Sudan. Subjects and Methods: Biopsies from 43 patients with nasopharyngeal carcinoma were examined for the presence of Epstein-Barr virus using EBER-ISH. Ten normal samples were used to assess the presence of the virus in non cancer tissues. Results: Fifty three samples were examined for the presence of the virus by EBER-ISH, 43 biopsies were NPC and ten were normal. Histologically the cases were, 20 (46.5%), 20 (46.5%) and 3 (7%) of the biopsies were classified as WHO types II, III and mixed type II and III, respectively;there were no cases of type I NPC. All nasopharyngeal carcinoma biopsies (100%) were positive for EBER1 in almost all carcinoma cells with focal and intense dark-blue staining limited to the nucleus;no hybridization was observed in the cytoplasm. No hybridization was observed in all ten non cancer tissues. Conclusion: All NPC cells are clearly EBV-infected. The virus is located in the nucleus of the tumour cells. The presence of Epstein-Barr virus in normal nasopharyngeal epithelia is not a common event. Objectives: The aim of this study is to detect Epstein-Barr virus (EBV) in nasopharyngeal carcinoma (NPC) biopsies of Sudanese patients using EBV-encoded RNA (EBER) in Situ hybridization (EBER-ISH). Study Design: This is a descriptive cross-sectional study conducted at the National Center for ENT diseases and Head and Neck Surgery and the Institute of Endemic Diseases, University of Khartoum, Khartoum City, Sudan. Subjects and Methods: Biopsies from 43 patients with nasopharyngeal carcinoma were examined for the presence of Epstein-Barr virus us