The LysR Transcription Factor, HexS, Is Required for Glucose Inhibition of Prodigiosin Production by <i>Serratia marcescens</i>
The LysR Transcription Factor, HexS, Is Required for Glucose Inhibition of Prodigiosin Production by <i>Serratia marcescens</i>作者机构：Charles T. Campbell Laboratory of Ophthalmic Microbiology Department of Ophthalmology University of Pittsburgh Pittsburgh USA College of Medicine University of Vermont Burlington USA
出 版 物：《Advances in Microbiology》 (微生物学（英文）)
年 卷 期：2012年第2卷第4期
页 面：511-517页
学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学]
主 题：Pigment Antibiotic Transcription Factor Secondary Metabolite
摘 要：Generation of many useful microbe-derived secondary metabolites, including the red pigment prodigiosin of the bacterium Serratia marcescens, is inhibited by glucose. In a previous report, a genetic approach was used to determine that glucose dehydrogenase activity (GDH) is required for inhibiting prodigiosin production and transcription of the prodigiosin biosynthetic operon (pigA-N). However, the transcription factor(s) that regulate this process were not characterized. Here we tested the hypothesis that HexS, a LysR-family transcription factor similar to LrhA of Escherichia coli, is required for inhibition of prodigiosin by growth in glucose. We observed that mutation of the hexS gene in S. marcescens allowed the precocious production of prodigiosin in glucose-rich medium conditions that completely inhibited prodigiosin production by the wild type. Unlike previously described mutants able to generate prodigiosin in glucoserich medium, hexS mutants exhibited GDH activity and medium acidification similar to the wild type. Glucose inhibittion of pigA expression was shown to be dependent upon HexS, suggesting that HexS is a key transcription factor in secondary metabolite regulation in response to medium pH. These data give insight into the prodigiosin regulatory pathway and could be used to enhance the production of secondary metabolites.
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