Characteristics of Archived Coagulase Negative <i>Staphylococci</i>Isolates at a University Hospital, Nairobi, Kenya

作     者：Shivachi Patricia Ingato Andrew Nyerere Kimang’a Geoffrey Omuse Samuel Kariuki Revathi Gunturu Victor Dinda 

作者机构：Jomo Kenyatta University of Agriculture and Technology Itromid KEMRI Nairobi Kenya Aga Khan University Hospital Nairobi Kenya Centres for Microbiology Research KEMRI Nairobi Kenya Masinde Muliro University of Science and Technology Nairobi Kenya 

出 版 物：《Open Journal of Medical Microbiology》 (医学微生物学（英文）)

年 卷 期：2014年第4卷第4期

页      面：236-241页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

主　　题：Coagulase Negative Staphylococcus (CoNS) Antimicrobial Susceptibility Patterns Methicillin Resistance 

摘      要：Background: Coagulase negative Staphylococci (CoNS) are normal inhabitants of the skin and mucous membranes and thus have been dismissed for a long time as culture contaminants even if they have been isolated from sterile specimens. The risk factors for CoNS infections include patients who are immunocompromised, implanted with foreign bodies or with indwelling devices. The aim of this study was to determine the antimicrobial susceptibility patterns and presence of mecA gene in methicillin resistant CoNS isolated in a teaching and referral hospital in Kenya. Methodology: This was a cross sectional retrospective study. Archived isolates were sub-cultured on 5% sheep blood agar. Speciation and antimicrobial susceptibility patterns were performed by Vitek2 technique. The presence of mecA gene was determined by (PCR). Results: A total of seven species were identified with Staphylococcus epidermidis having the highest percentage at 45.4% and Staphylococcus warneri with the lowest at 2.6%. High resistance to antibiotics that were tested was observed regardless of the source of the isolate. MecA gene was found in 90% of the isolates. Conclusion: Coagulase negative Staphylococci exhibited high levels of resistance generally. Most of the isolates carried the mecA gene. Despite some of the isolates being resistant to Cefoxitin, the mecA gene was not found. There is a possibility that methicillin resistance in these isolates is mediated using a different mechanism.