Engineering Chitosan Using <i>α</i>, <i>ω</i>-Dicarboxylic Acids—An Approach to Improve the Mechanical Strength and Thermal Stability

作     者：G. Sailakshmi Tapas Mitra Suvro Chatterjee A. Gnanamani 

作者机构：AU-KBC Research Centre MIT Anna University Chennai India. Microbiology Division CSIR-Central Leather Research Institute Chennai India 

出 版 物：《Journal of Biomaterials and Nanobiotechnology》 (生物材料与纳米技术（英文）)

年 卷 期：2013年第4卷第2期

页      面：151-164页

学科分类：07[理学] 0703[理学-化学] 

主　　题：Biomaterial Chitosan Cytocompatibility Dicarboxylic Acid Mechanical Property 

摘      要：The current scenario in tissue engineering research demands materials of requisite properties, viz., high porosity, mechanical stability, thermal stability, biocompatibility and biodegradability for clinical applications. However, bringing these properties in single biomaterial is a challenging task, which needs intensive research on suitable cross-linking agents. In the present study, 3D scaffold was prepared with above said properties using chitosan and oxalic (O), malonic (M), succinic (S), glutaric (G), adipic (A), pimelic (P), suberic (SU), azelaic (AZ) and sebacic (SE) acid (OMS- GAP-SAS) individually as a non covalent cross-linkers as well as the solvent for chitosan. Assessment on degree of cross-linking, mechanical strength, FT-IR analysis, morphological observation, thermal stability, binding interactions (molecular docking), in vitro biocompatibility and its efficacy as a wound dressing material were performed. Results revealed the degree of cross-linking for OMSGAP-SAS engineered chitosan were in the range between ≈55% - 65% and the biomaterial demonstrated thermal stability more than 300°C and also exhibited ≥3 - 4 fold increase in mechanical strength compared to chitosan alone. The bioinformatics studies evidently proved the chemistry behind the interaction of OMSGAP-SAS with chitosan. OMSGAP-SAS played dual role to develop the chitosan biomaterial with above said properties, thus matching the requirements needed for various applications.