Effect of Human Insulin on the Formation of Catheter-Associated <i>E. coli</i>Biofilms

作     者：Balbina J. Plotkin Zijian Wu Kathleen Ward Shaven Nadella Jacalyn M. Green Benny Rumnani 

作者机构：Department of Biochemistry Midwestern University Downers Grove USA Department of Microbiology and Immunology Midwestern University Downers Grove USA 

出 版 物：《Open Journal of Urology》 (泌尿学期刊（英文）)

年 卷 期：2014年第4卷第5期

页      面：49-56页

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Escherichia coli Insulin Catheters Diabetes Mellitus Biofilm Electronegativity 

摘      要：Biofilm formation is essential for the survival and growth of Escherichia coli?in catheter-associated infections. Individuals with type 2 diabetes mellitus can excrete insulin and/or glucose in their urine. This population also has an increased incidence of urinary tract infections. The focus of this study was to determine if the composition of Foley catheter material affects biofilm formation by E. coli in a model system for type 2 diabetes mellitus. Rubber (lubricious-coated), silicon-coated, silver-coated and nitrofurazone-coated catheter segments (5 mm;n = 6) were tested. Catheter segments were added to E. coli ATCC25922 (104 CFU/ml, final concentration) in artificial urine alone, or with insulin (40 μU/ml) and/or glucose (0.1%). After incubation (18 h, 37?C, in air and anaerobically) the level of catheter-associated biofilm was determined by crystal violet staining (Abs550nm). Statistical analysis was done by ANOVA with post-hoc analysis (Tukey). Neither nitrofurazone-coated nor silver-coated catheters supported the formation of E. coli biofilm, regardless of growth condition tested. In contrast, under aerobic biofilm formation on silicon catheters was significantly higher (p E. coli controls. Biofilm formation was also significantly increased