A simple method for the detection of hepatitis A virus in the environmental samples by polymerase chain reaction

作     者：Yang Feng, XuXun, Zhang Yusheng, Chen Rongzhong and Zhou Xiulan Third Institute of Oceanography, State Oceanic Administration, Xiamen 361005, China 

出 版 物：《Acta Oceanologica Sinica》 (海洋学报（英文版）)

年 卷 期：1993年第12卷第2期

页      面：279-284页

核心收录：

学科分类：07[理学] 0707[理学-海洋科学] 

主　　题：cloning RNA primer amplification contaminated satisfactory stranded visualize agarose RNase 

摘      要：Hepatitis A virus (HAV) is very persistent in the environment and is especially difficult to detect in the seafoods. In recent years, molecular cloning of the genome of HAV has led to sensitive polymerase chain reaction (PCR) method for the detection of HAV RNA. Here we describe a new and simple procedure to extract RNA from contaminated clams and the PCR method for detection of HAV in environmental samples. The specificity and efficiency of PCR amplification were studied using cDNA and RNA of HAV. Three primer couples gave satisfactory results. Some basic parameters of the PCR were modified to perform a highly specific and sensitive test.