Enzyme Dynamic Study on Calmodulin Ca^(++)-Mg^(++)-ATPase System of Red Blood Cells Inhibited byα-anordrin and Probimane

作     者：Lu Dayong Chen Enhong Cao Jingyi Xu Bin 

作者机构：School of Life SciencesShanghai University Shanghai Institute of Materia MedicaChinese Academy of Sciences 

出 版 物：《Advances in Manufacturing》 (先进制造进展(英文版))

年 卷 期：1998年第1期

页      面：84-86页

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

主　　题：antineoplastic agents calmodulin enzyme dynamics α anordrin probimane cell signal cancer pathology 

摘      要：To clarify the possible mechanisms of α anordin and probimane on calmodulin Ca ++ Mg ++ APTase system, enzyme dynamic study was carried out by determining three dynamic parameters [the substrate concentration(ATP) response curve, dose(inhibitors) response curve and time response curve]. Our data have shown that the inhibitory rates of α anordrin and probimane are unrelated to substrate(APT) concentrations, but related to calmodulin concentrations. The inhibition of α anordrin and probimane is very quick that is completed within 1 to 5 min and can maintain more than 1 hr in the same inhibitory rates. So it is possible that α anordrin and probimane are calmodulin competitors with calmodulin like binding whose actions can occur by affecting the reaction balance of substrate and product on target enzymes of calmodulin( Ca ++ Mg ++ ATPase).