Brain of the blind:transcriptomics of the golden-line cavefish brain

作     者：Fanwei MENG Yahui ZHAO Tom TITUS Chunguang ZHANG John H.POSTLETHWAIT 

作者机构：State Key Laboratory of Membrane BiologyInstitute of ZoologyChinese Academy of SciencesBeijing 100101China Key Laboratory of Zoological Systematics and EvolutionInstitute of ZoologyChinese Academy of SciencesBeijing 100101China Institute of NeuroscienceUniversity of OregonEugeneOR 97403USA 

出 版 物：《Current Zoology》 (动物学报（英文版）)

年 卷 期：2018年第64卷第6期

页      面：765-773页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 09[农学] 

基　　金：financially supported by grants from the National Natural Science Foundation of China NSFC31372191(F.W.M.),NSFC31471961 (Y.H.Z.) NIH grants NIH R01 OD011116(J.H.P.) R24RR032670 (J.H.P.) 

主　　题：cavefish cholesterol cyp46a optic tectum Sinocyclocheilus transcriptomics 

摘      要：The genus Sinocyclocheilus (golden-line barbel) includes 25species of cave-dwelling blind fish (cavefish)and more than 30 surface-dwelling species with normal *** environments are dark and generally nutrient-poor with few *** of several genera evolved convergent morphological adaptations in visual,pigmentation,brain,olfactory,and digestive systems. We compared brain morphology and gene expression patterns in a cavefish Sinocyclocheilus anophthalmus with those of a closely related surface-dwelling species *** showed that cavefish have a longer olfactory tract and a much smaller optic tectum than surface *** by RNA-seq revealed that many genes upregulated in cavefish are related to lysosomes and the degradation and metabolism of proteins,amino acids,and *** down- regulated in cavefish tended to involve activation of gene expression in cholesterol biosynthesis and cholesterol degradation in the *** encoding Srebfs (sterol regulatory element- binding transcription factors)and Srebf targets,including enzymes in cholesterol synthesis,were downregulated in cavefish brains compared with surface fish *** gene encoding Cyp46a1, which eliminates cholesterol from the brain,was also downregulated in cavefish brains,while the total level of cholesterol in the brain remained *** brains misexpressed several genes encoding proteins in the hypothalamus-pituitary axis,including Trh,Sst,Crh,Pomc,and *** results suggest that the rate of lipid biosynthesis and breakdown may both be depressed in golden-line cavefish brains but that the lysosome recycling rate may be increased in cavefish;properties that might be related to differences in nutrient availability in caves.