Glutathione S-transferase(GST) gene expression profiles in two marine bivalves exposed to BDE-47 and their potential molecular mechanisms

作     者：李斐 吴惠丰 王清 李雪花 赵建民 

作者机构：Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation Yantai Institute of Coastal Zone Research (YIC )Chinese Academy of Sciences Shandong Provincial Key Laboratory of Coastal Zone Environmental Processes Key Laboratory of Coastal Zone Environmental Processes and Ecological Remediation Yantai Institute of Coastal Zone Research (YIC) Chinese Academy of Sciences Key Laboratory of Industrial Ecology and Environmental Engineering (MOE) School of Environmental Science and TechnologyDalian University of Technology 

出 版 物：《Chinese Journal of Oceanology and Limnology》 (中国海洋湖沼学报（英文版）)

年 卷 期：2015年第33卷第3期

页      面：705-713页

核心收录：

学科分类：0908[农学-水产] 09[农学] 

基　　金：Supported by the National Natural Science Foundation of China(No.21107136) the International Foundation for Science(No.F/5230-1) 

主　　题：glutathione S-transferase (GST) Venerupis philippinarum Mytilus galloprovincialis 2,2',4,4'-tetrabromodiphenyl ether (BDE-47) molecular docking biomarker 

摘      要：Glutathione S-transferases （GSTs） are phase II enzymes that facilitate the detoxification of xenobioties and play important roles in antioxidant defense. We investigated the expression patterns of seven Venerupis philippinarum GSTs （VpGSTs） and four Mytilus galloprovincialis GSTs （MgGSTs） following exposure to BDE-47. Differential expressions of the seven VpGSTs and four MgGSTs transcripts were observed, with differences between the hepatopancreas and gills. Among these GSTs, the sigma classes （VpGSTS1, VpGSTS2, VpGSTS3, MgGST1, and MgGST3） were highly expressed in response to BDE-47 exposure, demonstrating their potential as molecular biomarkers for environmental biomonitoring studies. We obtained the three-dimensional crystal structures of VpGSTs and MgGSTs by homologous modeling. A model to elucidate the binding interactions between the ligands and receptors was defined by molecular docking, Hydrophobic and n were the most often observed interactions between BDE-47 and the GSTs.