Outer hair cells isolation from postnatal Sprague-Dawley rats

作     者：Mei-Hao Qi Yang Qiu Ke-Yong Tian Kun Liang Hui-Min Chang Ren-Feng Wang Er-Fang Chen Wei-Long Wang Ding-Jun Zha Jian-Hua Qiu Qi Mei-Hao;Qiu Yang;Tian Ke-Yong;Liang Kun;Chang Hui-Min;Wang Ren-Feng;Chen Er-Fang;Wang Wei-Long;Zha Ding-Jun;Qiu Jian-Hua

作者机构：Department of Otorhinolaryngology Head and Neck SurgeryXijing HospitalFourth Military Medical UniversityXi'anPR China Department of Otorhinolaryngology Head and Neck SurgeryTangdu HospitalFourth Military Medical UniversityXi'anPR China 

出 版 物：《World Journal of Otorhinolaryngology-Head and Neck Surgery》 (世界耳鼻咽喉头颈外科杂志（英文）)

年 卷 期：2019年第5卷第1期

页      面：14-18页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：the National Natural Science Foundation of China(NSFC 81120108008) 

主　　题：Outer hair cell Isolation technique Morphology Sprague-Dawley rat 

摘      要：Outer hair cells (OHCs) damage is a general phenomenon in clinical disorders such as noise-induced hearing loss and drug-induced hearing *** order to elucidate the mechanism underlying these disorders,OHCs-its diseased region needs to be deeply ***,OHCs array on the basilar membrane which contains massive cells with different ***,to isolate OHCs from this huge population is significant for revealing its pathological and molecular changes during disease *** the present study,we tried to isolate OHCs from the commonly used animal model-Sprague-Dawley (SD) *** separating outer hair cells from SD rats with different day ages,we found that 9 days after birth was a suitable time for the separation of the *** this time,the number of OHCs isolated from rats was large,and the cell morphology was typical of cylindrical *** isolated using this method are histologically suitable and quantitatively adequate for molecular biological and electrophysiological analyses.