3-Nitropropionic acid modifies neurotrophin mRNA expression in the mouse striatum: 18S-rRNA is a reliable control gene for studies of the striatum

作     者：S.Espíndola A Vilches-Flores E.Hernández-Echeagaray 

作者机构：Unidad de Biomedicina FES-I Universidad Nacional Autónoma de México Los Reyes IztacalaC. P. 54090 TlalnepantlaMéxico 

出 版 物：《Neuroscience Bulletin》 (神经科学通报（英文版）)

年 卷 期：2012年第28卷第5期

页      面：517-531页

核心收录：

学科分类：1002[医学-临床医学] 100204[医学-神经病学] 10[医学] 

基　　金：supported by the Consejo Nacional de Ciencia y Tecnología (Grant No. 42598) 

主　　题：neurotrophins striatum neurodegenerative disease PCR 18S 3-nitropropionic acid 

摘      要：Objective The aim of the present study was to determine the changes in the mRNA levels of neurotrophins and their receptors in the striatal tissue of mice treated with 3-nitropropionic acid （3-NP）. Methods At 1 and 48 h after the last drug administration, the mRNA expression of nerve growth factor, brain-derived neurotrophic factor, neurotrophin-3 and neurotrophin-4/5 as well as their receptors p75, TrkA, TrkB and TrkC, was evaluated using semi-quantitative （semi- Q） and real-time RT-PCR. β-actin mRNA and ribosomal 18S （18S rRNA） were tested as internal controls. Results 3-NP treatment did not affect mRNA expression of all neurotrophins and their respective receptors equally. Also, differences in neurotrophin and receptor mRNA expression were observed between semi-Q and real-time RT-PCR. Real-time RT-PCR was more accurate in evaluating the mRNA expression of the neurotrophins than semi-Q, and 18S rRNA was more reliable than β-actin as an internal control. Conclusion Neurotrophins and their receptors expression is differentially affected by neuronal damage produced by inhibition of mitochondrial respiration with 3-NP treatment in low, sub-chronic doses in vivo.