Benefits of the Combined Use of NSAID and Early Exercise on Tendon Healing in a Murine Model

作     者：Kelly Biancardini Gomes Barbato Clara Raposo Hanna Dias Renata Paiva Joao da Costa Libardo Rodriguez Guilherme de Almeida Oscar Rocha-Barbosa Liszt Palmeira de Oliveira Jorge Carvalho 

作者机构：School of MedicineState University of Rio de JaneiroRio de JaneiroBrazil Department of Surgical SpecialtiesSchool of MedicineState University of Rio de JaneiroRio de JaneiroBrazil Department of ZoologyRoberto Alcantara Gomes Institute of BiologyState University of Rio de JaneiroRio de JaneiroBrazil Department of Histology and EmbriologyRoberto Alcantara Gomes Institute of BiologyState University of Rio de JaneiroRio de JaneiroBrazil 

出 版 物：《Open Journal of Animal Sciences》 (动物科学期刊（英文）)

年 卷 期：2018年第8卷第4期

页      面：357-369页

学科分类：1002[医学-临床医学] 100214[医学-肿瘤学] 10[医学] 

基　　金：financial support from Fundacao de Amparo a Pesquisa do Estado do Rio de Janeiro-Faperj through scholarships to Clara Morena Abreu Raposo(2009 to 2011)and from grants to Liszt Palmeira de Oliveira 

主　　题：Achilles Tendon Rupture Experimental Study Non-Steroidal Anti-Inflammatory Drug Aerobic Exercise PCNA Biomechanics 

摘      要：Achilles tendon rupture is one of the most frequent tendon injuries. The aim of this experimental study was to evaluate the effect of a non-steroidal anti-inflammatory drug (NSAID) and of aerobic exercise, in an isolated fashion and combined, on cell proliferation and on the biomechanical aspects of the Achilles tendon during healing process after a complete tenotomy in 156 male Wistar rats. Half of the animals received an intra-muscular injection of tenoxicam for 7 days and exercise was initiated on the 8th day for half the animals of each group. Animals were sacrificed at 1, 2, 4 and 8 weeks after the tenotomy and cell proliferation was evaluated by immunohistochemistry for PCNA, biomechanical evaluation was performed with ultimate load and gait cycle analysis was also carried out. Animals were divided into four treatment groups: A) no NSAID and no exercise (control group);B) NSAID plus exercise;C) no NSAID, with exercise;D) NSAID and no exercise. We used the test of two-way analysis of variance with equal cells number, Kruskal-Wallis test and also, Bonferroni method, in the R Project program 3.5.0. The highest intensity of PCNA immunostaining was found at 2 weeks in all groups except for group A (control) that had the highest intensity at 1 week. Animals submitted to exercise had significantly higher (P = 0.02) ultimate loads when compared to animals that were not submitted to exercise at 8 weeks after injury. The animals that received NSAID presented with a more stable gait cycle than the animals that did not receive NSAID. Aerobic exercise, initiated early after a complete Achilles tendon tenotomy, was beneficial to the biomechanical aspects of the tendon that occur during tendon regeneration and the combined use of NSAID improved the gait’s characteristics which could be protective against re-ruptures.