Monitoring biofouling based on aerobic respiration in reverse osmosis system

作     者：Youngjae Yu Keun-Young Park Jaehyun Jung Wonjung Song Jaehyeok Kim Junhee Ryu Harshad Lade Jihyang Kweon 

作者机构：Department of Environmental EngineeringKonkuk University Department of Environment and EnergySejong University Department of Laboratory MedicineHallym University Medical Center 

出 版 物：《Journal of Environmental Sciences》 (环境科学学报（英文版）)

年 卷 期：2019年第31卷第4期

页      面：247-256页

核心收录：

学科分类：083002[工学-环境工程] 0830[工学-环境科学与工程（可授工学、理学、农学学位）] 08[工学] 

基　　金：supported by the National Research Foundation of Korea (NRF) grant funded by the Korean Government (MSIT) (No. 2018R1A2B2005745) 

主　　题：On-site monitoring Biofilm Aerobic respiration Resazurin reduction mechanism Resorufin fluorescence 

摘      要：A monitoring method of biofouling in reverse osmosis(RO) system was proposed based on the fluorescent signal of resorufin, which is reduced by nicotinamide adenine dinucleotide released from viable cells during aerobic respiration. The fluorescent signal of resorufin reduced by planktonic cells and microorganisms of biofilm showed linearity, indicating its feasibility to monitor biofouling in a RO system. For the application of the method to the lab-scale RO system, the injection concentration of resazurin and the injection flow rate were optimized. Biofilm on RO membranes continuously operated in a lab-scale RO system was estimated by resorufin fluorescence under optimized detection condition. As a result,resorufin fluorescence on RO membrane showed a significant increase in which the permeability of RO system decreased by 30.48%. Moreover, it represented the development of biofilm as much as conventional biofilm parameters such as adenosine triphosphate,extracellular polymeric substances, and biofilm thickness. The proposed method could be used as a sensitive and low-cost technology to monitor biofouling without autopsy of membranes.