Curcumin down-regulates PCNA,cyclin D1 and Bcl-X_L expression in human keratinocyte cell lines

作     者：Yang Zhengsheng Li Xiaoli Peng Zhenhui Song Jianwen Ren Jianwen 

作者机构：Department of Dermatology Second Affiliated Hospital Xi 'an Jiaotong University Xi 'an 710004 China 

出 版 物：《Journal of Medical Colleges of PLA(China)》 (中国人民解放军军医大学学报（英文版）)

年 卷 期：2010年第25卷第6期

页      面：321-330页

学科分类：0710[理学-生物学] 07[理学] 0905[农学-畜牧学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090501[农学-动物遗传育种与繁殖] 090102[农学-作物遗传育种] 

主　　题：Curcumin Keratinocyte Proliferating cell nuclear antigen Cyclin D1 Bcl-XL 

摘      要：Objective：To evaluate the effects of curcumin on regulating the proliferation,cell cycle distribution,apoptosis and relevant mechanisms in keratinocyte cell ***：The human immortalized human keratinocyte lines（HaCaT cells） were treated with different doses of *** effects of curcumin on cell viability were measured by MTT assay,and the cell cycle distribution and apoptosis determined by flow *** mRNA expression changes of proliferating cell nuclear antigen（PCNA）,cyclin D1 and Bcl-xL were from real-time PCR analysis and the protein levels were detected by Western ***：Data obtained in the study showed that curcumin could cause significantly inhibitory effect on proliferation in HaCaT cells in a time- and dose-dependent *** arrest at G1/S phase and significant apoptosis were observed after being treated with curcumin for 24 *** association with these,the expression of PCNA,cyclin D1 and Bcl-xL were decreased both at mRNA and protein levels for the same ***：Curcumin can inhibit proliferation,induce cell arrest at G1/S phase and cause apoptosis in HaCaT *** decreased expression of PCNA,cyclin D1 and Bcl-xL induced by curcumin contributes to the above effects in vitro.