Fsh-Pc-Sce complex mediates active transcription of Cubitus interruptus （Ci）

作     者：Xiangdong Lv Hao Chen Shuo Zhang Zhao Zhang Chenyu Pan Yuanxin Xia Jialin Fan Wenqing Wu Yi Lu Lei Zhang Hailong Wu Yun Zhao 

作者机构：State Key Laboratory of Cell Biology CAS Center for Excellence in Molecular Cell Science Innovation Center for Cell Signaling Network Shanghai Institute of Biochemistry and Cell Biology Chinese Academy of Sciences University of Chinese Academy of Sciences Shanghai 200031 China School of Life Science and Technology ShanghaiTech University Shanghai 201210 China 

出 版 物：《Journal of Molecular Cell Biology》 (分子细胞生物学报（英文版）)

年 卷 期：2018年第10卷第5期

页      面：437-447页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 0813[工学-建筑学] 0814[工学-土木工程] 

基　　金：This study was supported by grants from the National Natural Science Foundation of China (31630047  31671453  31771610)  the National Key Research and Development Program of China (2017YFA0503600)  and the 'Strategic Priority Research Program' of the Chinese Academy of Sciences (XDB19020100) and was sponsored by the Program of Shanghai Academic/Technology Research Leader (17XD1404100)  the International Partnership Program of Chinese Academy of Sciences (153D31KYSB20160137)  and the 'Cross and Cooperation in Science and Technology Innovation Team' Project of the Chinese Academy of Sciences (173176001000163307) 

主　　题：Ci Fsh Hedgehog polycomb complex Sce transcriptional activation 

摘      要：The Hedgehog （Hh） signaling pathway plays important roles in both embryonic development and adult tissue homeostasis. Such biological functions are mediated by the transcription factor Cubitus interruptus （Ci）. Yet the transcriptional regulation of the effector Ci itself is poorly investigated. Through an RNAi-based genetic screen, we identified that female sterile （1） homeotic （Fsh）, a transcription co-activator, directly activates Ci transcription. Biochemistry assays demonstrated physical interactions among Fsh, Sex combs extra （Sce）, and Polycomb （Pc）. Functional assays further showed that both Pc and Sce are required for Ci expression, which is not likely mediated by the derepression of Engraited （En）, a repressor of Ci, in Pc or Sce mutant cells. Finally, we provide evidence showing that Pc/Sce facilitates the binding of Fsh at Ci locus and that the physical interaction between Fsh and Pc is essential for Fsh-mediated Ci transcription. Taken together, we not only uncover that Ci is transcriptionally regulated by Fsh-Pc-Sce complex but also provide evidence for the coordination between Fsh and PcG proteins in transcriptional regulation.