Efficient management and maintenance of ultrasonic nebulizers to prevent microbial contamination
Efficient management and maintenance of ultrasonic nebulizers to prevent microbial contamination作者机构：Clinical LaboratoryKyorin University Hospital Department of Microbiology and ImmunologyGraduate School of Health Care SciencesTokyo Medical and Dental University Department of Laboratory MedicineKyorin University School of Medicine Department of General MedicineKyorin University School of Medicine
出 版 物：《World Journal of Methodology》 (世界方法学杂志)
年 卷 期：2016年第6卷第1期
页 面：126-132页
学科分类：07[理学] 0701[理学-数学] 070101[理学-基础数学]
主 题：Prevention Contamination Burkholderia cepacia Randomly amplified polymorphic DNA assay Ultrasonic nebulizer
摘 要：AIM:To seek the cause of Burkholderia cepacia complex(Bcc) infection outbreak and evaluate the efficacy of new methods for nebulizer maintenance. METHODS: We investigated the annual number of Bcc isolates recovered from clinical samples in our hospital between 1999 and 2013. Swab samples were randomly collected for bacterial culture before patient use from 10 each of the two machine types in August 2001; these included 20 samples from each of the following: Drain tubes, operating water chambers, oscillators, and nebulizing chambers. In addition, 10 samples each of nebulizer solutions before and after use were cultured. For environmental investigation, 10 samples were collected from sinks in the nurse stations of the wards where patients positive for Bcc were hospitalized. Numbers of Bcc isolates were compared before and after introduction of new methods for nebulizer maintenance in October 2001. In addition, randomly amplified polymorphic DNA(RAPD) assay was applied to find the genetic divergence of the Bcc isolates obtained from clinical samples and nebulizers. RESULTS: From January 1999 to December 2013, a total of 487 Bcc isolates were obtained from clinical specimens from 181 patients. Notably, 322(66.1%) Bcc isolates were obtained from clinical specimens from 1999 to 2001, including 244(115 patients) from sputum and 34(11 patients) from blood. During this period, 14 isolates were obtained from nebulizer components. Among these, six were derived from nebulizer drain tubes, five from operating water chambers, and one from the oscillator before patient use, and two from nebulizer solutions after patient use. When Bcc was isolated from the nebulizer solution after patient use, Bcc was simultaneously detected in other parts of the nebulizer. Bcc was not isolated from any nebulizer solution before use. RAPD assays revealed similar DNA profiles in isolates obtained from patients and nebulizers. Investigation revealed damaged diaphragms in many nebulizers. The new main
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