Identification of New Resistance Loci Against Sheath Blight Disease in Rice Through Genome-Wide Association Study

作     者：CHEN Zongxiang FENG Zhiming KANG Houxiang ZHAO Jianhua CHEN Tianxiao LI Qianqian GONG Hongbing ZHANG Yafang CHEN Xijun PAN Xuebiao LIU Wende WANG Guoliang ZUO Shimin 

作者机构：Jiangsu Key Laboratory of Crop Genetics and Physiology/Co-Innovation Center for Modern Production Technology of Grain Crops Yangzhou University State Key Laboratory for Biology of Plant Diseases and Insect Pests/Institute of Plant Protection Chinese Academy of Agricultural Sciences Zhenjiang Agricultural Research Institute 

出 版 物：《Rice science》 (水稻科学（英文版）)

年 卷 期：2019年第26卷第1期

页      面：21-31页

核心收录：

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 09[农学] 0901[农学-作物学] 0836[工学-生物工程] 0902[农学-园艺学] 

基　　金：partially supported by the Open Funding from State Key Laboratory for Biology of Plant Diseases and Insect Pests(Grant No.SKLOF201403) by the Natural Science Foundation of China(Grant Nos.31571748 and 31701057) the Natural Science Foundation of Jiangsu Province,China(Grant Nos.BK20171293 and BK20141291) 

主　　题：genome-wide association study quantitative trait locus rice sheath blight plant height 

摘      要：Sheath blight(SB) caused by the soil borne pathogen Rhizoctonia solani is one of the most serious global rice diseases. Breeding resistant cultivar is the most economical and effective strategy to control the disease. However, no rice varieties are completely resistant to SB, and only a few reliable quantitative trait loci(QTLs) linked with SB resistance have been identified to date. In this study, we conducted a genome-wide association study(GWAS) of SB resistance using 299 varieties from the rice diversity panel 1(RDP1) that were genotyped using 44 000 high-density single nucleotide polymorphism(SNP) markers. Through artificial inoculation, we found that only 36.5% of the tested varieties displayed resistance or moderate resistance to SB. In particular, the aromatic and aus sub-populations displayed higher SB resistance than the tropical japonica(TRJ), indica and temperate japonica sub-populations. Seven varieties showed similar resistance levels to the resistant control YSBR1. GWAS identified at least 11 SNP loci significantly associated with SB resistance in the three independent trials, leading to the identification of two reliable QTLs, qSB-3 and qSB-6, on chromosomes 3 and 6. Using favorable alleles or haplotypes of significantly associated SNP loci, we estimated that both QTLs had obvious effects on reducing SB disease severity and can be used for enhancing SB resistance, especially in improving SB resistance of TRJ sub-population rice varieties. These results provided important information and genetic materials for developing SB resistant varieties through breeding.