Antidiabetic effect of combination of fractionated-extracts of Andrographis paniculata and Centella asiatica: In vitro study

作     者：LOM.Fitrawan R.Ariastuti RR.TjANDrawinata AE.Nugroho S.Pramono 

作者机构：Master Program of Pharmaceutical Sciences of Faculty of Pharmacy Universitas Gadjah Mada Faculty of Pharmacy Universitas Halu Oleo Dexa Laboratories of Biomolecular Sciences Department of Pharmacology and Clinical Pharmacy Faculty of Pharmacy Universitas Gadjah Mada Department of Pharmaceutical Biology Faculty of Pharmacy Universitas Gadjah Mada 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2018年第8卷第11期

页      面：527-532页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：supported by the Ministry of Research and Technology and Higher Education of the Republic of Indonesia(RISTEKDIKTI)through "Hibah Unggulan Perguruan Tinggi 2017" 

主　　题：Andrographis paniculata Centella asiatica GLUT4 PPAR γ 3T3-L1 

摘      要：Objective: To examine the effect of combination of Andrographis paniculata herb fraction(AHF) and Centella asiatica herb fraction(CHF) on PPAR γ and GLUT4 mRNA expression in 3 T3-L1 adipocyte, and its effect on insulin-stimulated glucose uptake. Methods: 3 T3 L1 adipocyte cells were used to investigate gene expression of PPARy reverse transcription-polymerase chain reaction method. The γ and GLUT4 protein b adipocyte cells were differentiated by using insulin, dexamethasone and 3-isobutyl-1-methylxanthine from 3 T3-L1 cells. Pioglitazone, AHF, CHF and the combination of both herbs were evaluated on glucose uptake activity, PPAR γ and GLUT4 mRNA expressions in 3 T3-L1 adipocyte. Results: The results showed that combination of AHF at 30 μg/mL and CHF at 10 μg/mL could enhance insulin-stimulated glucose uptake. The combination also increased PPAR γ and GLUT4 mR NA expressions significantly in comparison to those of negative control(DMSO). These effects were equal in comparison to those of pioglitazone(0.02 μM) and its single extracts Conclusions: The combination of AHF and CHF can increase glucose uptake and insulin sensitivity through up-regulation of PPAR γ and GLUT4 mRNA expressions in 3 T3-L1 adipocyte.