Spectroscopic investigation on the telomeric DNA base sequence repeat

作     者：Sichuan Xu Xicheng Ai Zhaoyong Sun Qiyuan Zhang Xingkang Zhang 

作者机构：Chinese Acad Sci Inst Chem State Key Lab Struct Chem Unstable & Stable Speci Ctr Mol Sci Beijing 100080 Peoples R China 

出 版 物：《Chinese Science Bulletin》 (CHINESE SCIENCE BULLETIN)

年 卷 期：2002年第47卷第2期

页      面：108-111页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 

基　　金：Thiswork was supported by the Science Creative Project of the Chinese Academy of Sciences (Grant No. KJCX2-H2) 

主　　题：DNA telomere oligonucleotide fluorescence quantum yield. 

摘      要：Telomeres are protein-DNA complexes at the terminals of linear chromosomes, which protect chromoso-mal integrity and maintain cellular replicative capacity. From single-cell organisms to advanced animals and plants, structures and functions of telomeres are both very conser-vative. In cells of human and vertebral animals, telomeric DNA base sequences all are (TTAGGG)n. In the present work, we have obtained absorption and fluorescence spectra measured from seven synthesized oligonucleotides to simu-late the telomeric DNA system and calculated their relative fluorescence quantum yields on which not only telomeric DNA characteristics are predicted but also possibly the shortened telomeric sequences during cell division are im-plied. Oligonucleotide 5’- TTAGGGTTAGGG holds a low relative fluorescence quantum yield and remarkable excitation energy innerconversion, which tallies with the telomeric sequence of (TTAGGG)n. This result shows that telomeric DNA has a strong non-radiative or innerconvertible capabil-