Early monitoring of response to antimetabolic treatment of gemcitabine:A comparison of ^(18)F-FLT and ^(18)F-FDG uptake in Patu 8988 human pancreatic carcinoma cells

作     者：DENG Shengming ZHANG Bin WU Yiwei ZHANG Wei 

作者机构：Department of Nuclear Medicine No.1 Hospital Affiliated to Sooehow University Suzhou 215006 China 

出 版 物：《Nuclear Science and Techniques》 (核技术（英文）)

年 卷 期：2011年第22卷第5期

页      面：293-298页

核心收录：

学科分类：0710[理学-生物学] 07[理学] 0905[农学-畜牧学] 071009[理学-细胞生物学] 09[农学] 0901[农学-作物学] 090102[农学-作物遗传育种] 

基　　金：Supported by Key Laboratory Program of Nuclear Medicine Ministry of Health of China(Grant No.KF200904) 

主　　题：细胞摄取 早期预测 治疗效果 FDG 癌细胞 胰腺癌 LSF 药物敏感性 

摘      要：It is essential to predict the treatment efficacy of pancreatic carcinoma *** purpose of this study was to examine whether ^(18)F-FDG(2 -deoxy-2 -[^(18)F]fiuoro-D-glucose) or ^(18)F-FLT(3 -deoxy-3 -^(18)F-fluorothymidine) PET can be used for chemosensitivity testing by investigating the binding characteristic of ^(18)F-FDG or ^(18)F-FLT with Patu 8988 human pancreatic carcinoma cell and the influence of gemcitabine in the uptake of ^(18)F-FDG or ^(18)F-FLT on Patu *** the conditions of 1×10~6 cells,3.7 kBq ^(18)F-FDG or ^(18)F-FLT,and incubation at 37℃for 100 min,the cell uptake of ^(18)F-FDG and ^(18)F-FLT was(60.60±3.05)%and(50.57±2.81)%,*** was a significant decrease in TKl-LI(thymidine kinase 1 labeling index) 24 h after administration of *** uptakes of ^(18)F-FDG and ^(18)F-FLT were negatively correlated with the doses of gemcitabine(r= -0.928 for ^(18)F-FDG,r= -0.876 for ^(18)F-FLT,P0.01).When same doses of gemcitabine were administered,the ^(18)F-FLT uptake inhibition rate was significantly higher than that of ^(18)F-FDG(P0.01).These results indicate that the response to gemcitabine could be predicted as early as 24 h by ^(18)F-FDG or ^(18)F-FLT PET scans.^(18)F-FLT is more sensitive than ^(18)F-FDG to predict the response to therapy.