Region-specific microRNA signatures in the human epididymis

作     者：James A Browne Shih-Hsing Leir Scott E Eggener Ann Harris James A Browne;Shih-Hsing Leir;Scott E Eggener;Ann Harris

作者机构：Department of Genetics and Genome SciencesCase Western Reserve UniversityClevelandOH 44106USA Human Molecular Genetics ProgramLurie Children＇s Research CenterChicagoIL 60614USA Department of PediatricsNorthwestern University Feinberg School of MedicineChicagoIL 60611USA Section of UrologyUniversity of Chicago Medical CenterChicagoIL 60611USA. 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2018年第20卷第6期

页      面：539-544页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 080402[工学-测试计量技术及仪器] 0804[工学-仪器科学与技术] 

基　　金：supported by the NIH (PI: AH) 

主　　题：caput cauda corpus human epididymis microRNA 

摘      要：The epithelium of the human epididymis maintains an appropriate luminal environment for sperm maturation that is essential for male fertility. Regional expression of small noncoding RNAs such as microRNAs contributes to segment-specific gene expression and differentiated functions. MicroRNA profiles were reported in human epididymal tissues but not specifically in the epithelial cells derived from those regions. Here, we reveal miRNA signatures of primary cultures of caput, corpus, and cauda epididymis epithelial cells and of the tissues from which they were derived. We identify 324 epithelial cell-derived microRNAs and 259 tissue-derived microRNAs in the epididymis, some of which displayed regionalized expression patterns in cells and/or tissues. Caput cell-enriched miRNAs included miR-573 and miR-155. Cauda cell-enriched miRNAs included miR-1204 and miR-770. Next, we determined the gene ontology pathways associated with in silico predicted target genes of the differentially expressed miRNAs. The effect of androgen receptor stimulation on miRNA expression was also investigated. These data show novel epithelial cell-derived miRNAs that may regulate the expression of important gene networks that are responsible for the regionalized gene expression and function of the epididymis.