Inhibiting severe acute respiratory syndrome-associated coronavirus by small interfering RNA

作     者：张仁礼 郭中敏 陆家海 孟锦绣 周灿权 詹希美 黄冰 余新炳 黄民 潘兴华 凌文华 陈系古 万卓越 郑焕英 鄢心革 王一飞 冉延超 刘新健 马俊鑫 王承宇 张必良 

作者机构：Sun Yat-Sen University Center for Disease Control and Prevention of Guangdong Province Jinan University Maxim BiotechInc. The Quartermaster University of PLA Medical School of University of Massachusetts 

出 版 物：《Chinese Medical Journal》 (中华医学杂志（英文版）)

年 卷 期：2003年第116卷第8期

页      面：1262-1264页

核心收录：

学科分类：1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 100401[医学-流行病与卫生统计学] 10[医学] 

主　　题：Animals Cercopithecus aethiops RNA, Small Interfering SARS Virus Transfection Vero Cells Virus Replication 

摘      要：OBJECTIVE: To evaluate the effectiveness of small interfering RNA (siRNA) on inhibiting severe acute respiratory syndrome (SARS)-associated coronavirus replication, and to lay bases for the future clinical application of siRNA for the treatment of viral infectious diseases. METHODS: Vero-E6 cells was transfected with siRNA before SARS virus infection, and the effectiveness of siRNA interference was evaluated by observing the cytopathic effect (CPE) on Vero-E6 cells. RESULTS: Five pairs of siRNA showed ability to reduce CPE dose dependently, and two of them had the best effect. CONCLUSION: siRNA may be effective in inhibiting SARS-associated coronavirus replication.