Focused transcription from the human CR2/CD21 core promoter is regulated by synergistic activity of TATA and Initiator elements in mature B cells

作     者：Rhonda L Taylor Mark N Cruickshank Mahdad Karimi Han Leng Ng Elizabeth Quail Kenneth M Kaufman John B Harley Lawrence J Abraham Betty P Tsao Susan A Boackle Daniela Ulgiati 

作者机构：School of Pathology and Laboratory Medicine Centre for Genetic Origins of Health and Disease The University of Western Australia Crawley WA Australia Biochemistry and Molecular Biology School of Chemistry and Biochemistry The University of Western Australia Crawley WA Australia Telethon Kids Institute The University of Western Australia Crawley WA Australia Cincinnati Children's Hospital Medical Center Cincinnati OH USA US Department of Veterans Affairs Medical Center Cincinnati OH USA Division of Rheumatology Department of Medicine University of California at Los Angeles Los Angeles CA USA Division of Rheumatology University of Colorado School of Medicine Aurora CO USA 

出 版 物：《Cellular & Molecular Immunology》 (中国免疫学杂志（英文版）)

年 卷 期：2016年第13卷第1期

页      面：119-131页

核心收录：

学科分类：0710[理学-生物学] 071010[理学-生物化学与分子生物学] 1004[医学-公共卫生与预防医学(可授医学、理学学位)] 1002[医学-临床医学] 1001[医学-基础医学(可授医学、理学学位)] 07[理学] 09[农学] 0902[农学-园艺学] 090202[农学-蔬菜学] 

基　　金：supported by the National Health and Medical Research Council of Australia the Lupus Research Institute (to SAB) the Alliance for Lupus Research (to SAB, DU and BPT) 美国国立卫生研究院(NIH)项目 the US Department of Veterans Affairs (to JBH) 

主　　题：B cells core promoter CR2/CD21 molecular biology transcription factor 

摘      要：Complement receptor 2 （CR2/CD21） is predominantly expressed on the surface of mature B cells where it forms part of a coreceptor complex that functions, in part, to modulate B-cell receptor signal strength. CR2/CD21 expression is tightly regulated throughout B-cell development such that CR2/CD21 cannot be detected on pre-B or terminally differentiated plasma cells. CR2/CD21 expression is upregulated at B-cell maturation and can be induced by IL-4 and CD40 signaling pathways. We have previously characterized elements in the proximal promoter and first intron of CR2/CD21 that are involved in regulating basal and tissue-specific expression. We now extend these analyses to the CR2/CD21 core promoter. We show that in mature B cells, CR2/~D21 transcription proceeds from a focused TSS regulated by a non-consensus TATA box, an initiator element and a downstream promoter element. Furthermore, occupancy of the general transcriptional machinery in pre-B versus mature B-cell lines correlate with CR2/CD21 expression level and indicate that promoter accessibility must switch from inactive to active during the transitional B-cell window.