Antiretroviral naive and treated patients: Discrepancies of B cell subsets during the natural course of human immunodeficiency virus type 1 infection

作     者：Olga Tsachouridou Lemonia Skoura Pantelis Zebekakis Apostolia Margariti Adamantini Georgiou Dimitrios Bougiouklis Dimitrios Pilalas Antonios Galanos Michael Daniilidis Symeon Metallidis 

作者机构：1~(st) Internal Medicine Department Infectious Diseases Division Medical School Aristotle University of Thessaloniki National AIDS Reference Centre of Northern Greece Medical School Aristotle University of Thessaloniki 

出 版 物：《World Journal of Virology》 (世界病毒学杂志)

年 卷 期：2016年第5卷第4期

页      面：155-160页

学科分类：10[医学] 

主　　题：B cell subpopulations Time-trend Memory cells Human immunodeficiency virus infection Highly active antiretroviral therapy 

摘      要：AIM To evaluate alterations of memory B cell subpopulations during a 48-wk period in human immunodeficiency virus type 1(HIV-1) patients. METHODS Forty-one antiretroviral na?ve and 41 treated HIV-1 patients matched for age and duration of HIV infection were recruited. All clinical, epidemiological and laboratory data were recorded or measured. The different B cell subsets were characterized according to their surface markers: Total B cells(CD19^+), memory B cells(CD19^+CD27^+, BMCs), resting BMCs(CD19^+CD27^+CD21^(high), RM), exhausted BMCs(CD19^+CD21^(low)CD27-, EM), IgM memory B(CD19^+CD27^+IgM^(high)), isotype-switched BMCs(CD19^+CD27^+IgM-, ITS) and activated BMCs(CD19^+CD21^(low+)CD27^+, AM) at baseline on week 4 and week 48.RESULTS Mean counts of BMCs were higher in treated patients. There was a marginal upward trend of IgM memory B cell proportions which differed significantly in the treated group(overall trend, P = 0.004). ITS BMC increased over time significantly in all patients. Naive patients had of ^(low)er levels of EM B cells compared to treated, with a downward trend, irrespectively of ^(high)ly active antiretroviral therapy(HAART) intake. Severe impairment of EM B cells was recorded to both treated(P = 0.024) and naive(P = 0.023) and patients. Higher proportions of RM cells were noted in HAART group, which differed significantly on week 4^(th)(P = 0.017) and 48th(P = 0.03). Higher levels of AM were preserved in HAART naive group during the whole study period(week 4: P = 0.018 and 48: P = 0.035). HIV-RNA viremia strongly correlated with AM B cells(r = 0.54, P = 0.01) and moderately with RM cells(r =-0.45, P = 0.026) at baseline.CONCLUSION HIV disrupts memory B cell subpopulations leading to impaired immunologic memory over time. BMC, RM, EM and ITS BMC were higher in patients under HAART. Activated BMCs(AM) were higher in patients without HAART. Viremia correlated with AM and RM. Significant depletion was recorded in EM B cells irrespectively of HAART intake. Perturbations in BMC-populations are not fully restored by antiretrovirals.