Protective effects of erythropoietin against acute lung injury in a rat model of acute necrotizing pancreatitis

作     者：Oge Tascilar Güldeniz Karadeniz Cakmak Ishak Ozel Tekin Ali Ugur Emre Bulent Hamdi Ucan Burak Bahadir Serefden Acikgoz Oktay Irkorucu Kemal Karakaya Hakan Balbaloglu Gürkan Kertis Handan Ankarali Mustafa Comert 

作者机构：Department of SurgeryZonguldak Karaelmas UniversitySchool of MedicineKozlu-Zonguldak 67600Turkey Department of ImmunologyZonguldak Karaelmas UniversitySchool of MedicineKozlu-Zonguldak 67600Turkey Department of PathologyZonguldak Karaelmas UniversityThe School of MedicineKozlu-Zonguldak 67600Turkey Department of BiochemistryZonguldak Karaelmas UniversitySchool of MedicineKozlu-Zonguldak 67600Turkey Department of BiostatisticsZonguldak Karaelmas UniversitySchool of MedicineKozlu-Zonguldak 67600Turkey 

出 版 物：《World Journal of Gastroenterology》 (世界胃肠病学杂志（英文版）)

年 卷 期：2007年第13卷第46期

页      面：6172-6182页

核心收录：

学科分类：1002[医学-临床医学] 100201[医学-内科学(含：心血管病、血液病、呼吸系病、消化系病、内分泌与代谢病、肾病、风湿病、传染病)] 10[医学] 

主　　题：Erythropoietin Acute pancreatitis Acute lunginjury Acute respiratory distress syndrome Cytokine 

摘      要：AIM： TO investigate the effect of exogenous erythro- poietin （EPO） administration on acute lung injury （ALI） in an experimental model of sodium taurodeoxycholate- induced acute necrotizing pancreatitis （ANP）. METHODS： Forty-seven male Wistar albino rats were randomly divided into 7 groups： sham group （n = 5）, 3 ANP groups （n = 7 each） and 3 EPO groups （n = 7 each）. ANP was induced by retrograde infusion of 5% sodium taurodeoxycholate into the common bile duct. Rats in EPO groups received 1000 U/kg intramuscular EPO immediately after induction of ANP. Rats in ANP groups were given 1 mL normal saline instead. All animals were sacrificed at postoperative 24 h, 48 h and 72 h. Serum arnilase, IL-2, IL-6 and lung tissue malondialdehyde （MDA） were measured. Pleural effusion volume and lung/body weight （LW/BW） ratios were calculated. Tissue levels of TNF-a, IL-2 and IL-6 were screened immunohistochemically. Additionally, ox-LDL accumulation was assessed with immune-fluorescent staining. Histopathological alterations in the lungs were also ***： The mean pleural effusion volume, calculated LW/BW ratio, serum IL-6 and lung tissue MDA levels were significantly lower in EPO groups than in ANP groups. No statistically significant difference was observed in either serum or tissue values of IL-2 among the groups. The level of tumor necrosis factor-（~ （TNF-（~） and IL-6 and accumulation of ox-LDL were evident in the lung tissues of ANP groups when compared to EPO groups, particularly at 72 h. Histopathological evaluation confirmed the improvement in lung injury parameters a^er exogenous EPO administration, particularly at 48 h and 72 h. CONCLUSION： EPO administration leads to a significant decrease in ALI parameters by inhibiting polymorphonuclear leukocyte （PMNL） accumulation, decreasing the levels of proinflammatory cytokines in circulation, preserving microvascular endothelial cell integrity and reducing oxidative stress-associated lipid peroxidation and therefore, can