Pressurized Liquid Extraction of Pigments from Chlamydomonas *** Chemical Characterization by HPLC–MS/MS

作     者：Lidia Montero Mahshid Sedghi Yolanda García Carlos Almeida Carl Safi Nicole Engelen‑Smit Alejandro Cifuentes Jose A.Mendiola Elena Ibáñez 

作者机构：Applied Analytical ChemistryUniversity of Duisburg-EssenUniversitaetsstr.545141 EssenGermany Laboratory of FoodomicsBioactivity and Food Analysis DepartmentInstitute of Food Science Research CIAL(CSIC-UAM)Campus de CantoblancoC/Nicolás Cabrera 928049 MadridSpain Spanish Bank of AlgaeInstituto de Oceanografía y Cambio GlobalFundación Canaria Parque Científico‑TecnológicoUniversidad de Las Palmas de Gran CanariaMuelle de Taliarte s/n35214 Telde(Canary Islands)Spain Wageningen Food and Biobased ResearchWageningen University and ResearchPO Box 176700 AA WageningenThe Netherlands 

出 版 物：《Journal of Analysis and Testing》 (分析检测（英文）)

年 卷 期：2018年第2卷第2期

页      面：149-157页

核心收录：

学科分类：1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：EU MIRACLES project(Seventh Framework Programme KBBE-Grant Agreement No.613588 Multi-product Integrated bioRefinery of Algae:from Carbon dioxide and Light Energy to high-value Specialties) 

主　　题：Antioxidant capacity Chlamydomonas sp. HPLC–DAD–APCI–MS/MS Pigments Pressurized liquid extraction Carotenoids 

摘      要：Pressurized liquid extraction using green solvents(ethanol and water)was applied to extract photosynthetic pigments from the microalgae Chlamydomonas *** experimental design is employed to determine the effect of the extraction conditions on the extraction yield and the antioxidant capacity of the different extracts determined using 1,1-diphenyl-2-picryl-hydrazyl and TEAC *** the extracts show significant antioxidant activity in all ***,in this work,the carotenoid and chlorophyll composition of the Chlamydomonas extracts was analyzed by HPLC–DAD–APCI–MS/MS for the first time,being the main compounds lutein and pheophythin a′.