Evaluation of corporal fibrosis in cadaveric pericardium and vein grafts for tunica albuginea substitution in rats

作     者：Somboon Leungwattanakij Vaewvadee Tiewthanom Wayne J.G. Hellstrom SOMBOON LEUNGWATTANAKIJ;VAEWVADEE TIEWTHANOM;WAYNE J.G. HELLSTROMDEPARTMENT OF UROLOGY;DEPARTMENT OF NURSING;RAMATHIBODI HOSPITAL;MAHIDOL UNIVERSITY;BANGKOK;THAILAND DEPARTMENT OF UROLOGY;TULANE UNIVERSITY HEALTH SCIENCES CENTER;NEW ORLEANS;LOUISIANA;USA

作者机构：Department of Urology Ramathibodi Hospital Mahidol University Bangkok 10400 ThailandDepartment of Nursing Ramathibodi Hospital Mahidol University Bangkok 10400 ThailandDepartment of Urology Tulane University Health Sciences Center New Orleans Louisiana USA 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2003年第5卷第4期

页      面：295-299页

核心收录：

学科分类：1002[医学-临床医学] 08[工学] 080501[工学-材料物理与化学] 0805[工学-材料科学与工程（可授工学、理学学位）] 080502[工学-材料学] 

主　　题：Peyronie's disease pericardial graft vein graft 

摘      要：Abstract Aim: To evaluate the degree of corporal fibrosis in rats with cadaveric pericardium or vein as grafting materials for tunica albuginea substitution. Materials and methods: Thirty male Sprague-Dawley rats (300 g-325 g) were divided at random into 3 groups of 10 animals each: group 1 was the sham-operated controls and groups 2 and 3 underwent wedge excision of tunica albuginea and replacement with cadaveric pericardium and vein grafts, respectively. Four months later, rats were sacrificed and the penis removed to assess the degree of fibrosis using RT PCR technique for TGP-β1 mRNA expression. The tissues were fixed in 10 % formalin, paraffin-embedded and stained with Masson s trichrome and Verhoff s van Giesen for collagen and elastic fibers. Results: Four months after grafting, there was minimal fibrosis surrounding the patch in the vein graft rats and moderate fibrosis in the pericardial graft rats. The degree of penile fibrosis in the pericardial graft rats was significantly higher than that in the controls (P0.05). Conclusion: The degree of penile fibrosis of cadaveric pericardial graft was significantly higher than that of the control group, while in the vein graft group it was comparable to the latter. The authors believe that the vein graft may be a more ideal substance to be used as the tunica albuginea substitute than the pericardial graft in the surgical treatment of Peyronie s disease.