Quantitative estimation of hesperidin by HPTLC in different varieties of citrus peels

作     者：Prawez Alam Aftab Alam Khalid Anwer Saleh I Alqasoumi 

作者机构：Department of PharmacognosyCollege of PharmacySalman Bin Abdulaziz University Department of PharmaceuticsCollege of PharmacySalman Bin Abdulaziz University Department of PharmacognosyCollege of PharmacyKing Saud University 

出 版 物：《Asian Pacific Journal of Tropical Biomedicine》 (亚太热带生物医学杂志（英文版）)

年 卷 期：2014年第4卷第4期

页      面：262-266页

学科分类：0710[理学-生物学] 0831[工学-生物医学工程（可授工学、理学、医学学位）] 1008[医学-中药学(可授医学、理学学位)] 1006[医学-中西医结合] 100602[医学-中西医结合临床] 10[医学] 

基　　金：Supported by Deanship of Scientific Research Salman Bin Abdulaziz University Al-Kharj KSA(Grant No.33/S/54) 

主　　题：HPTLC Citrus Hesperidin 

摘      要：Objective:To develop a simple,selective,sensitive and accurate high-performance thin layer chromatography(HPTLC)method to determine the quantity of hesperidin in different varieties of citrus ***:The method was carried out in aluminum-backed silica gel 60 F_(254)plates with ethyl acetate-methanol-water 15:3:2(%,v/v)as mobile ***:A compact band was obtained for hesperidin at R_f value of(0.40±0.04).The calibration plot was linear in the range of 100-800 ng/spot of hesperidin and the correlation coefficient of 0.9986was indicative of good linear dependence of peak area on *** of detection(8.87ng/spot),limit of quantification(23.21 ng/spot),accuracy(less than 2%)and recovery(ranging from98.55-99.38)were found ***:The method developed can be used for routine analysis of hesperidin in crude drug as well as in herbal and pharmaceutical dosage form containing citrus fruits as an ingredient.