Effect of surgical procedures on prostate tumor gene expression profiles

作     者：lie Li Zhi-Hong Zhang Chang-lun Yin Christian Pavlovich Jun Luo Robert Getzenberg Wei Zhang 

作者机构：Department of UrologyThe First Affiliated Hospital of Nanjing Medical UniversityNanjing 210029China Department of PathologyThe First Affiliated Hospita Medical UniversityNanjing 210029China Department of UrologyThe Johns Hopkins University School of MedicineBaltimoreMD 21287USA 

出 版 物：《Asian Journal of Andrology》 (亚洲男性学杂志（英文版）)

年 卷 期：2012年第14卷第5期

页      面：708-714页

核心收录：

学科分类：0710[理学-生物学] 090601[农学-基础兽医学] 071010[理学-生物化学与分子生物学] 081704[工学-应用化学] 07[理学] 08[工学] 0817[工学-化学工程与技术] 09[农学] 0906[农学-兽医学] 

基　　金：国家自然科学基金 

主　　题：CO2 insufflation expression microarray laparoscopic radical prostatectomy open radical prostatectomy prostate cancer stress response 

摘      要：Current surgical treatment of prostate cancer is typically accomplished by either open radical prostatectomy （ORP） or robotic-assisted laparoscopic radical prostatectomy （RALRP）. Intra-operative procedural differences between the two surgical approaches may alter the molecular composition of resected surgical specimens, which are indispensable for molecular analysis and biomarker evaluation. The objective of this study is to investigate the effect of different surgical procedures on RNA quality and genome-wide expression signature. RNA integrity number （RIN） values were compared between total RNA samples extracted from consecutive LRP （n= 11） and ORP （n= 24） prostate specimens. Expression profiling was performed using the Agilent human whole-genome expression microarrays. Expression differences by surgicat type were analyzed by Volcano plot analysis and gene ontology analysis. Quantitative reverse transcription （RT）-PCR was used for expression validation in an independent set of LRP （n=8） and ORP （n=8） samples. The LRP procedure did not compromise RNA integrity. Differential gene expression by surgery types was limited to a small subset of genes, the number of which was smaller than that expected by chance. Unexpectedly, this small subset of differentially expressed genes was enriched for those encoding transcription factors, oxygen transporters and other previously reported surgery-induced stress-response genes, and demonstrated unidirectional reduction in LRP specimens in comparison to ORP specimens. The effect of the LRP procedure on RNA quality and genome-wide transcript levels is negligible, supporting the suitability of LRP surgical specimens for routine molecular analysis. Blunted in vivo stress response in LRP specimens, likely mediated by CO2 insufflation but not by longer ischemia time, is manifested in the reduced expression of stress-response genes in these specimens.